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Similarity of protein encoded by the human c-erb-B-2 gene to epidermal growth factor receptor

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Abstract

A novel v-erb-B-related gene, c-erb-B-2, which has been identified in the human genome1,2, maps to human chromosome 17 at q21 (ref. 40), and seems to encode a polypeptide with a kinase domain that is highly homologous with, but distinct from, that of the epidermal growth factor (EGF) receptor1. The c-erb-B-2 gene is conserved in vertebrates and it has been suggested1 that the neu gene, detected in a series of rat neuro/glioblastomas3, is, in fact, the rat c-erb-B-2 gene. Amplification of the c-erb-B-2 gene in a salivary adenocarcinoma and a gastric cancer cell line MKN-7 suggests that its over-expression is sometimes involved in the neoplastic process. To determine the nature of the c-erb-B-2 protein, we have now molecularly cloned complementary DNA for c-erb-B-2 messenger RNA prepared from MKN-7 cells. Its sequence shows that the c-erb-B-2 gene encodes a possible receptor protein and allows an analysis of the similarity of the protein to the EGF receptor and the neu product. As a consequence of chromosomal aberration in MKN-7 cells, a 4.6-kilobase (kb) normal transcript and a truncated 2.3-kb transcript of c-erb-B-2 are synthesized at elevated levels. The latter transcript presumably encodes only the extracellular domain of the putative receptor.

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Author notes

  1. Tetsu Akiyama: Meiji College of Pharmacy, 1-35-23, Nozawa, Setagaya-ku, Tokyo 154, Japan.

  2. Nobuo Nomura: Nippon Medical School at Sakuragi, 1-10-19, Uenosakuragi, Taito-ku, Tokyo 110, Japan.

Authors and Affiliations

  1. Institute of Medical Science, University of Tokyo, 4-6-1, Shirokanedai, Minato-ku, Tokyo, 108, Japan

    Tadashi Yamamoto, Shuntaro Ikawa, Tetsu Akiyama, Kentaro Semba, Nobuo Nomura, Nobuyuki Miyajima, Toshiyuki Saito & Kumao Toyoshima

Authors
  1. Tadashi Yamamoto
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  2. Shuntaro Ikawa
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  3. Tetsu Akiyama
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  4. Kentaro Semba
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  5. Nobuo Nomura
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  6. Nobuyuki Miyajima
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  7. Toshiyuki Saito
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  8. Kumao Toyoshima
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Yamamoto, T., Ikawa, S., Akiyama, T. et al. Similarity of protein encoded by the human c-erb-B-2 gene to epidermal growth factor receptor. Nature 319, 230–234 (1986). https://doi.org/10.1038/319230a0

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  • DOI: https://doi.org/10.1038/319230a0

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