Skip to main content

Advertisement

SpringerLink
Log in

Reconstitution of functional receptor for human interleukin-2 in mouse cells

  • Letter
  • Published:

From Nature

View current issue Submit your manuscript

Abstract

Interleukin-2 (IL-2) has a key role in the antigen-specific clonal growth of T lymphocytes, by virtue of its interaction with a specific cell-surface receptor (IL-2R)1–4. The growth signal seems to be delivered by IL-2 bound to the high-affinity, but not the low-affinity, receptor5,6. Genes encoding IL-2 (refs 7–13) and its receptor (that is, Tac-antigen)14–17 have been cloned and analysed in detail. We have now achieved cell-type-specific reconstitution of the high-affinity human IL-2R by expressing the complementary DNA cloned from normal lymphocytes. A mouse T-lymphocytic line, EL-4, expressed human IL-2R with high (dissociation constant (Kd) = 160–220 pM) and low (Kd = 2.1–2.2 nM) affinity for recombinant human IL-2, while mouse L929 cells expressed only a single class of the IL-2R with lower affinity (Kd = 34.5 nM) for the ligand. We also show that the human IL-2R expressed in EL-4 cells responds to IL-2 and mediates reversed signal transduction: growth of the EL-4 cells harbouring the IL-2 R is inhibited specifically by human recombinant IL-2. The approach described here may provide a general experimental framework for elucidating the molecular basis of signal transduction mediated by specific receptor–ligand interaction.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Smith, K. A. A. Rev. Immun. 2, 319–334 (1984).

    Article  CAS  Google Scholar 

  2. Robb, R. J., Munck, A. & Smith, K. A. J. exp. Med. 154, 1455–1474 (1981).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  3. Cantrell, D. A. & Smith, K. A. J. exp. Med. 158, 1895–1911 (1983).

    Article  CAS  PubMed  Google Scholar 

  4. Smith, K. A. & Cantrell, D. A. Proc. natn. Acad. Sci. U.S.A. 82, 864–868 (1985).

    Article  ADS  CAS  Google Scholar 

  5. Robb, R. J., Greene, W. C. & Rusk, C. M. J. exp. Med. 160, 1126–1146 (1984).

    Article  CAS  PubMed  Google Scholar 

  6. Uchiyama, T. et al. J. clin. Invest. 76, 446–453 (1985).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  7. Taniguchi, T. et al. Nature 302, 305–310 (1983).

    Article  ADS  CAS  PubMed  Google Scholar 

  8. Holbrook, N. J. et al. Proc. natn. Acad. Sci. U.S.A. 81, 1634–1638 (1984).

    Article  ADS  CAS  Google Scholar 

  9. Devos, R. et al. Nucleic Acids Res. 11, 4307–4323 (1983).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  10. Fujita, T., Takaoka, C., Matsui, H. & Taniguchi, T. Proc. natn. Acad. Sci. U.S.A. 80, 7437–7441 (1983).

    Article  ADS  CAS  Google Scholar 

  11. Kashima, N. et al. Nature 313, 402–404 (1985).

    Article  ADS  CAS  PubMed  Google Scholar 

  12. Yokota, T. et al. Proc. natn. Acad. Sci. U.S.A. 82, 68–72 (1985).

    Article  ADS  CAS  Google Scholar 

  13. Fuse, A. et al. Nucleic Acids Res. 12, 9323–9331 (1984).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  14. Leonard, W. J. et al. Nature 311, 626–631 (1984).

    Article  ADS  CAS  PubMed  Google Scholar 

  15. Nikaido, T. et al. Nature 311, 631–635 (1984).

    Article  ADS  CAS  PubMed  Google Scholar 

  16. Cosman, D. et al. Nature 312, 768–771 (1984).

    Article  ADS  CAS  PubMed  Google Scholar 

  17. Shimizu, A. et al. Nucleic Acids Res. 13, 1505–1516 (1985).

    Article  CAS  Google Scholar 

  18. Taniguchi, T., Pang, R. H. L., Yip, Y. K., Henriksen, D. & Vilcek, J. Proc. natn. Acad. Sci. U.S.A. 78, 3469–3472 (1981).

    Article  ADS  CAS  Google Scholar 

  19. Uchiyama, T., Broder, S. & Waldmann, T. A. J. Immun. 126, 1393–1397 (1981).

    CAS  PubMed  Google Scholar 

  20. Uchiyama, T., Nelson, D. L., Fleischer, T. A. & Waldmann, T. A. J. Immun. 126, 1398–1403 (1981).

    CAS  PubMed  Google Scholar 

  21. Gorman, C. M., Merlino, G. T., Willingham, M. C., Pastan, I. & Howard, B. H., Proc. Natn. Acad. Sci. U.S.A. 79, 6777–6781 (1982).

    Article  ADS  CAS  Google Scholar 

  22. Mulligan, R. C. & Berg, P. Science 209, 1422–1427 (1980).

    Article  ADS  CAS  PubMed  Google Scholar 

  23. Farrar, J. J. et al. Immun. Rev. 63, 129–166 (1982).

    Article  CAS  PubMed  Google Scholar 

  24. Gillis, S., Perm, M. M., Ou, W. & Smith, K. A. J. Immun. 120, 2027–2032 (1978).

    CAS  PubMed  Google Scholar 

  25. Osawa, H. & Diamantstein, T. J. Immun. 132, 2445–2450 (1984).

    CAS  PubMed  Google Scholar 

  26. Gill, G. N. & Lazar, C. S. Nature 293, 305–307 (1981).

    Article  ADS  CAS  PubMed  Google Scholar 

  27. Buss, J. E., Kudlow, J. E., Lazar, C. S. & Gill, G. N. Proc. natn. Acad. Sci. U.S.A. 79, 2574–2578 (1982).

    Article  ADS  CAS  Google Scholar 

  28. Roberts, A. B. et al. Proc. natn. Acad. Sci. U.S.A. 82, 119–123 (1985).

    Article  ADS  CAS  Google Scholar 

  29. Sugamura, K., Nakai, S., Fujii, M. & Hinuma, Y. J. exp. Med. 161, 1243–1248 (1985).

    Article  CAS  PubMed  Google Scholar 

  30. Farrar, W. L. & Anderson, W. B. Nature 315, 233–235 (1985).

    Article  ADS  CAS  PubMed  Google Scholar 

  31. Greene, W. C. et al. J. exp. Med. 162, 363–368 (1985).

    Article  CAS  PubMed  Google Scholar 

  32. Oi, V. T., Morrison, S. L., Herzenberg, L. A. & Berg, P. Proc. natn. Acad. Sci. U.S.A. 80, 825–829 (1983).

    Article  ADS  CAS  Google Scholar 

  33. Chu, G. & Sharp, P. Gene 13, 197–202 (1981).

    Article  CAS  PubMed  Google Scholar 

  34. Parks, D. R., Bryan, V. M., Oi, V. T. & Herzenberg, L. A. Proc. natn. Acad. Sci. U.S.A. 76, 1962–1966 (1979).

    Article  ADS  CAS  Google Scholar 

Download references

Author information

Author notes

  1. Takashi Uchiyama: Department of Internal Medicine, Faculty of Medicine, Kyoto University, Sakyo-ku, Kyoto 606, Japan

Authors and Affiliations

  1. Institute for Molecular and Cellular Biology, Osaka University, Suita-shi, Osaka, 565, Japan

    Masanori Hatakeyama, Seijiro Minamoto, Takashi Uchiyama, Richard R. Hardy, Gen Yamada & Tadatsugu Taniguchi

Authors
  1. Masanori Hatakeyama
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Seijiro Minamoto
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Takashi Uchiyama
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Richard R. Hardy
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Gen Yamada
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Tadatsugu Taniguchi
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Hatakeyama, M., Minamoto, S., Uchiyama, T. et al. Reconstitution of functional receptor for human interleukin-2 in mouse cells. Nature 318, 467–470 (1985). https://doi.org/10.1038/318467a0

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/318467a0

  • Springer Nature Limited

This article is cited by

Search

Navigation