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Abrogation of IL-3 and IL-2 dependence by recombinant murine retroviruses expressing v-myc oncogenes

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Abstract

Several oncogenes are thought to cause transformation by affecting the signal transmission pathway of growth factors. One example is the induction of c-myc, the cellular homologue of the avian transforming oncogene v-myc, by platelet-derived growth factor (PDGF)among a set of genes associated with competence induction in fibroblasts1,2. Another of the competence genes, r-fos, has been shown to be related to v-fos, the transforming gene of the FBJ sarcoma virus3. In addition, PDGF induces c-fos, the cellular homologue of v-fos4–6. The importance of c-myc induction is suggested by the observation that c-myc, under the control of a glucocorticoid regulator, can partially relieve the requirement of fibroblasts for PDGF7. We have examined the effects of oncogenes on haematopoietic/lymphoid cell differentiation, immortalization and factor dependence for growth. Here we report the effects of recombinant murine retroviruses capable of expressing the avian v-myc. With interleukin-3 (IL-3)- or interleukin-2 (IL-2)-dependent cells, the viruses abrogated the requirement for growth factors and suppressed c-myc expression.

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Authors and Affiliations

  1. Laboratory of Viral Carcinogenesis, National Cancer Institute, Frederick, Maryland, 21701, USA

    U. R. Rapp & J. L. Cleveland

  2. Department of Molecular Biology and Biochemistry, University of California, Irvine, California, 92717, USA

    K. Brightman

  3. NCI-Frederick Cancer Research Facility, Litton Bionetics Inc., Basic Research Program, Frederick, Maryland, 21701, USA

    A. Scott & J. N. Ihle

Authors
  1. U. R. Rapp
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  2. J. L. Cleveland
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  3. K. Brightman
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  4. A. Scott
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  5. J. N. Ihle
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Rapp, U., Cleveland, J., Brightman, K. et al. Abrogation of IL-3 and IL-2 dependence by recombinant murine retroviruses expressing v-myc oncogenes. Nature 317, 434–438 (1985). https://doi.org/10.1038/317434a0

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  • DOI: https://doi.org/10.1038/317434a0

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