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Location of functional regions of acetylcholine receptor α-subunit by site-directed mutagenesis

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Abstract

The availability of cloned cDNAs encoding the four subunits of the Torpedo acetylcholine receptor, which can be expressed to make functional receptors in Xenopus oocytes, has made possible a detailed investigation of the functions of the different structural components of the receptor. The functional analysis of receptors with α-subunits altered at specific sites by site-directed mutagenesis of the cDNA has allowed the location of specific regions of the α-subunit molecule involved in acetylcholine binding and forming a transmembrane ionic channel.

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Authors and Affiliations

  1. Department of Medical Chemistry, Kyoto University Faculty of Medicine, Kyoto, 606, Japan

    Masayoshi Mishina, Takamasa Tobimatsu, Keiji Imoto, Ken-ichi Tanaka, Yoshihiko Fujita, Kazuhiko Fukuda, Masaaki Kurasaki, Hideo Takahashi, Yuuki Morimoto & Shosaku Numa

  2. Department of Physiology, Kyoto University Faculty of Medicine, Kyoto, 606, Japan

    Tomoyuki Takahashi & Motoy Kuno

  3. Pharmaceutical Institute, Keio University School of Medicine, Tokyo, 160, Japan

    Tadaaki Hirose & Seiichi Inayama

Authors
  1. Masayoshi Mishina
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  2. Takamasa Tobimatsu
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  3. Keiji Imoto
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  4. Ken-ichi Tanaka
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  5. Yoshihiko Fujita
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  6. Kazuhiko Fukuda
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  7. Masaaki Kurasaki
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  8. Hideo Takahashi
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  9. Yuuki Morimoto
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  10. Tadaaki Hirose
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  11. Seiichi Inayama
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  12. Tomoyuki Takahashi
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  13. Motoy Kuno
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  14. Shosaku Numa
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Mishina, M., Tobimatsu, T., Imoto, K. et al. Location of functional regions of acetylcholine receptor α-subunit by site-directed mutagenesis. Nature 313, 364–369 (1985). https://doi.org/10.1038/313364a0

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  • DOI: https://doi.org/10.1038/313364a0

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