Abstract
‘Identifier’ or ID sequences are present in 62% of the RNA polymerase II and III transcripts made in vitro from brain nuclei but in fewer than 4% of the transcripts made from the nuclei of other tissues. An homologous 160-nucleotide cytoplasmic poly(A)+ RNA species, BC1, and a smaller species, BC2, are located in vivo exclusively in neural tissues. Cloned ID sequences are polymerase III templates in vitro. Our data suggest a model in which brain-specific polymerase III transcription of ID sequences located in introns of brain genes activates those genes in a primary manner for polymerase II transcription.
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Sutcliffe, J. G., Milner, R. J., Bloom, F. E. & Lerner, R. A. Proc. natn. Acad. Sci. U.S.A. 79, 4942–4946 (1982).
Barta, A., Richards, R. I., Baxter, J. D. & Shine, J. Proc. natn. Acad. Sci. U.S.A. 78, 4867–4871 (1981).
Hojvat, S., Baker, G., Kirsteins, L. & Lawrence, A. M. Brain Res. 239, 543–557 (1982).
Milner, R. J., Bloom, F. E., Lai, C., Lerner, R. A. & Sutcliffe, J. G. Proc. natn. Acad. Sci. U.S.A. (in the press).
Thomas, P. Proc. natn. Acad. Sci. U.S.A. 77, 5201–5205 (1980).
Rigby, P. W. J., Dieckmann, M., Rhodes, C. & Bery, P. J. Molec. Biol. 113, 237–251 (1977).
Reddy, R. et al. J. biol. Chem. 256, 8452–8457 (1981).
Jelinek, W. & Leinwand, L. Cell 15, 205–214 (1978).
Milner, R. J. & Sutcliffe, J. G. Nucleic Acids Res. 11, 5497–5520 (1983).
Galli, G., Hofstetter, H. & Birnstiel, M. L. Nature 294, 626–631 (1981).
Weil, P. A., Luse, D. S., Segall, J. & Roeder, R. G. Cell 18, 469–484 (1979).
Brown, D. D. & Gurdon, J. B. Proc. natn. Acad. Sci. U.S.A. 79, 2064–2068 (1977).
Sutcliffe, J. G. Cold Spring Harb. Symp. quant. Biol. 43, 77–90 (1978).
Bogenhagen, D. F. & Brown, D. D. Cell 24, 261–270 (1981).
Manley, J. L., Fine, A., Cano, A., Sharp, P. A. & Gefter, M. L. Proc. natn. Acad. Sci. U.S.A. 77, 3855–3859 (1980).
Sudgen, B. & Keller, W. J. biol. Chem. 248, 3777–3788 (1973).
Spadafora, C., Ondet, P. & Chambon, P. Eur. J. Biochem. 100, 225–235 (1979).
Weisbrod, S. & Weintraub, H. Proc. natn. Acad. Sci. U.S.A. 76, 631–635 (1979).
Manley, J. L. & Colozzo, M. T. Nature 300, 376–379 (1982).
Sutcliffe, J. G., Milner, R. J., Shinnick, T. M. & Bloom, F. E. Cell 33, 671–682 (1983).
Levy, D. E., Lerner, R. A. & Wilson, M. C. Proc. natn. Acad. Sci. U.S.A. 79, 5823–5827 (1982).
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Sutcliffe, J., Milner, R., Gottesfeld, J. et al. Identifier sequences are transcribed specifically in brain. Nature 308, 237–241 (1984). https://doi.org/10.1038/308237a0
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DOI: https://doi.org/10.1038/308237a0
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