Abstract
We have cloned and sequenced a human mRNA specific for mammalian T-lymphoid cells. The message was found to be expressed in human and murine T lymphoblasts, thymocytes and phytohaemagglutinin-stimulated T lymphocytes. The protein deduced from the cDNA sequence has a molecular weight of 34,938 and shows extensive similarity to the entire length of the variable, joining and constant regions of mammalian immunoglobulin light chains. In addition, the relative positions of the cysteine residues are similar to those of the light chains of murine and human immunoglobulin molecules. These properties suggest that the cDNA clone may correspond to a message that specifies part of the human T-cell receptor.
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Porter, R. R. Science 180, 713–716 (1973).
Edelman, G. M. Science 180, 830–840 (1973).
Hozumi, N. & Tonegawa, S. Proc. natn. Acad. Sci. U.S.A. 73, 3628–3632 (1976).
Honjo, T. An. Rev. Immun. 1, 499–528 (1983).
Rabbits, T. H., Bentley, D. L. & Milstein, C. P. Immun. Rev. 59, 69–91 (1981).
Tonegawa, S. Nature 302, 575–581 (1983).
Capra, J. D. & Kehoe, J. M. Adv. Immun. 20, 1–40 (1975).
Eichmann, K. Adv. Immun. 26, 195–254 (1978).
Janemay, C. A. & Jason, J. M. CRC Crit. Rev. Immun. 1, 133–164 (1980).
Miller, J. F. A. P., Marohan, G. & Walker, I. D. Immun. Today 4, 141–143 (1983).
Allison, J. P., McIntyre, B. W. & Bloch, D. J. Immun. 129, 2293–2300 (1982).
Haskins, K. et al. J. exp. Med. 157, 1149–1169 (1983).
Meuer, S. C. et al. J. exp. Med. 157, 705–719 (1983).
Acuto, O. et al. Cell 34, 717–726 (1983).
Kappler, J. et al. Cell 34, 727–737 (1983).
McIntyre, B. W. & Allison, J. P. Cell 34, 739–746 (1983).
Minawada, J., Okamura, T. & Moore, G. E. J. natn. Cancer Inst. 49, 891–895 (1972).
Nagasawa, K. & Mak, T. W. Cell. Immun. 71, 396–403 (1982).
Delia, D., Greaves, M. F., Newman, R. A. & Sutherland, D. R. Int. J. Cancer 29, 23–31 (1982).
Land, H. et al. Nucleic Acids Res. 9, 2251–2266 (1981).
Clark, S. P. & Mak, T. W. Nucleic Acids Res. 10, 3315–3330 (1982).
Parnes, J. R. et al. Proc. natn. Acad. Sci. U.S.A. 78, 2253–2257 (1981).
Sanger, F., Nicklen, S. & Coulson, A. R. Proc. natn Acad. Sci. U.S.A. 74, 5463–5467 (1977).
Clark, S. P. & Mak, T. W. Proc. natn. Acad. Sci. U.S.A. 80, 5037–5041 (1983).
Bothwell, A. L. M. et al. Nature 298, 380–382 (1982).
Hieter, P. A., Hollis, G. F., Korsmeyer, S. J., Waldmann, T. A. & Leder, P. Nature 294, 536–540 (1981).
Heiter, P. A., Max, E. E., Seidman, J. G., Maizel, J. V. Jr & Leder, P. Cell 22, 197–207 (1980).
Matthyssens, G. & Rabbitts, T. H. Proc. natn. Acad. Sci. U.S.A. 77, 6561–6565 (1980).
Bernando, O. & Gough, N. M. Proc. natn. Acad. Aci. U.S.A. 77, 3634–3636 (1980).
Bregegere, A. J. P. et al. Nature 292, 78–81 (1981).
Moriuchi, T., Chang, H. C., Denome, R. & Silver, J. Nature 301, 80–82 (1983).
Malissen, M., Malissen, B. & Jordan, B. R. Proc. natn. Acad. Sci. U.S.A. 79, 893–897 (1982).
Chechik, B. S. & Minowada, J. J. natn. Cancer Inst. 63, 609–614 (1979).
Reinherz, E. L. & Schlossman, S. F. Cell 19, 821–827 (1980).
Alt, F. et al. Cell 20, 293–301 (1980).
Rogers et al. Cell 20, 303–312 (1980).
Rigby, P. W. J., Dieckmann, M., Rhodes, C. & Berg, P. J. molec. Biol. 113, 237–251 (1977).
Chirgwin, J. M., Przybyla, A. E., MacDonald, R. J. & Rutter, W. J. Biochemistry 18, 5294–5299 (1979).
Thomas, P. S. Proc. natn. Acad. Sci. U.S.A. 77, 5201–5205 (1980).
Laemmli, U. K. Nature 277, 680–685 (1970).
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Yanagi, Y., Yoshikai, Y., Leggett, K. et al. A human T cell-specific cDNA clone encodes a protein having extensive homology to immunoglobulin chains. Nature 308, 145–149 (1984). https://doi.org/10.1038/308145a0
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DOI: https://doi.org/10.1038/308145a0
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