Abstract
Transgenic mice were produced by microinjection of a rearranged, functional immunoglobulin κ gene into fertilized mouse eggs and implantation of the microinjected embryos into foster mothers. Mice that integrated the injected gene were mated and the DNA, RNA and serum κ chains of their offspring were analysed. The data from offspring of three different transgenic mice indicate that the microinjected gene is expressed in the spleen, but not the liver of mice which inherited the injected gene.
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Sakano, H., Huppi, K., Heinrich, G. & Tonegawa, S. Nature 280, 288–294 (1979).
Max, E., Seidman, J. & Leder, P. Proc. natn. Acad. Sci. U.S.A. 76, 3450–3454 (1979).
Brinster, R. L., Chen, H. Y. & Trumbauer, M. E. Science 211, 396–398 (1981).
Brinster, R. L. et al. Cell 27, 223–231 (1981).
Palmiter, R. D., Chen, H. Y. & Brinster, R. L. Cell 29, 701–710 (1982).
Palmiter, R. D. et al. Nature 300, 611–615 (1982).
Walfield, A. M., Storb, U., Selsing, E. & Zentgraf, H. Nucleic Acids Res. 8, 4689–4707 (1980).
Walfield, A., Selsing, E., Arp, B. & Storb, U. Nucleic Acids Res. 9, 1101–1109 (1981).
Kabat, E. A., Wu, T. T. & Bilofsky, H. in Sequences of Immunoglobulin Chains, NIH Publ. No. 80-2008 (1979).
Potter, M. Adv. Immun. 25, 141–207 (1977).
Selsing, E. & Storb, U. Nucleic Acids Res. 9, 5725–5735 (1981).
Storb, U., Arp, B. & Wilson, R. Nucleic Acids Res. 8, 4681–4687 (1980).
Storb, U., Near, R., Putnam, D. & Clagett, J. Molec. Immun. 17, 947–957 (1980).
Storb, U., Hager, L., Wilson, R. & Putnam, D. Biochemistry 16, 5432–5438 (1977).
O'Farrell, P. H. J. biol. Chem. 250, 4007–4021 (1975).
Wagner, T. E. et al. Proc. natn. Acad. Sci. U.S.A. 78, 6376–6380 (1981).
Constantini, F. & Lacy, E. Nature 294, 92–94 (1981).
Wagner, E. R., Stewart, T. A. & Mintz, B. Proc. natn. Acad. Sci. U.S.A. 78, 5016–5020 (1981).
Storb, U., Wilson, R., Selsing, E. & Walfield, A. Biochemistry 20, 990–996 (1981).
Storb, U. & Arp, B. Proc. natn. Acad. Sci. U.S.A. (in the press).
Perry, R. P. et al. Proc. natn. Acad. Sci. U.S.A. 77, 1937–1941 (1980).
Hieter, P. A., Max, E. E., Seidman, J. G., Maizel, J. V. Jr & Leder, P. Cell 22, 197–207 (1980).
Parslow, T. G. & Granner, D. K. Nature 299, 449–451 (1982).
Boss, M. A. Nature 303, 281–282 (1982).
Queen, C. & Baltimore, D. Cell 33, 741–748 (1983).
Emorine, L., Kuehl, M., Weir, L., Leder, P. & Max, E. Nature 304, 447–449 (1983).
Gillies, S. D., Morrison, S. L., Oi, V. T. & Tonegawa, S. Cell 33, 717–728 (1983).
Banerji, J., Olson, L. & Schaffner, W. Cell 33, 729–740 (1983).
Mercola, M., Wang, X., Olsen, J. & Calame, K. Science 221, 663–665 (1983).
Perry, R. P., Kelley, D. E., Coleclough, C. & Kearney, J. F. Proc. natn. Acad. Sci. U.S.A. 78, 247–251 (1981).
Southern, E. M. J. molec. Biol. 98, 503–517 (1975).
McMaster, G. K. & Carmichael, G. G. Proc. natn. Acad. Sci. U.S.A. 64, 4835–4839 (1977).
Thomas, P. S. Proc. natn. Acad. Sci. U.S.A. 77, 5201–5205 (1981).
Denhardt, D. Biochem. biophys. Res. Commun. 23, 641–652 (1966).
Merrill, C. R., Goldman, D., Sedman, S. A. & Ebert, M. H. Science 211, 1437–1438 (1981).
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Brinster, R., Ritchie, K., Hammer, R. et al. Expression of a microinjected immunoglobulin gene in the spleen of transgenic mice. Nature 306, 332–336 (1983). https://doi.org/10.1038/306332a0
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DOI: https://doi.org/10.1038/306332a0
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