Skip to main content
SpringerLink
Log in

Release of Ca2+ from a nonmitochondrial intracellular store in pancreatic acinar cells by inositol-1,4,5-trisphosphate

  • Letter
  • Published:

From Nature

View current issue Submit your manuscript

Abstract

Activation of receptors for a wide variety of hormones and neurotransmitters leads to an increase in the intracellular level of calcium. Much of this calcium is released from intracellular stores but the link between surface receptors and this internal calcium reservoir is unknown. Hydrolysis of the phosphoinositides, which is another characteristic feature of these receptors1–3, has been implicated in calcium mobilization1. The primary lipid substrates for the receptor mechanism seem to be two polyphosphoinositides, phosphatidylinositol 4-phosphate (PtdIns4P) and phosphatidylinositol 4,5-bisphosphate (PtdIns4,5P2), which are rapidly hydrolysed following receptor activation in various cells and tissues4–10. The action of phospholipase C on these polyphosphoinositides results in the rapid formation of the water-soluble products inositol 1,4-bisphosphate (Ins1,4P2) and inositol 1,4,5-trisphosphate (Ins1,4,5P3)9,11,12. In the insect salivary gland, where changes in Ins1,4P2 and Ins1,4,5P3 have been studied at early time periods, increases in these inositol phosphates are sufficiently rapid to suggest that they might mobilize internal calcium12. We report here that micromolar concentrations of Ins1,4,5P3 release Ca2+ from a nonmitochondrial intracellular Ca2+ store in pancreatic acinar cells. Our results strongly suggest that this is the same Ca2+ store that is released by acetylcholine.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Michell, R. H. Biochim. biophys. Acta 415, 81–147 (1975).

    Article  CAS  Google Scholar 

  2. Berridge, M. J. Molec. cell. Endocr. 24, 115–140 (1981).

    Article  CAS  Google Scholar 

  3. Hokin, M. R. & Hokin, L. E. J. biol. Chem. 203, 967–977 (1953).

    CAS  Google Scholar 

  4. Abdel-Latif, A. A., Akhtar, R. A. & Hawthorne, J. N. Biochem. J. 162, 61–73 (1977).

    Article  CAS  Google Scholar 

  5. Kirk, C. J., Creba, J. A., Downes, P. & Michell, R. H. Biochem. Soc. Trans. 9, 377–379 (1981).

    Article  CAS  Google Scholar 

  6. Thomas, A. P., Marks, J. S., Coll, K. E. & Williamson, J. R. J. biol. Chem. 258, 5716–5725 (1983).

    CAS  PubMed  Google Scholar 

  7. Weiss, S. J., McKinney, J. S. & Putney, J. W. Biochem. J. 206, 555–560 (1982).

    Article  CAS  Google Scholar 

  8. Billah, M. M. & Lapetina, E. G. J. biol. Chem. 257, 12705–12708 (1982).

    CAS  PubMed  Google Scholar 

  9. Agranoff, B. W., Murthy, P. & Seguin, E. B. J. biol. Chem. 258, 2076–2078 (1983).

    CAS  Google Scholar 

  10. Putney, J. W., Burgess, G. M., Halenda, S. P., McKinney, J. S. & Rubin, R. P. Biochem. J. 212, 483–488 (1983).

    Article  CAS  Google Scholar 

  11. Berridge, M. J., Dawson, R. M. C., Downes, C. P., Heslop, J. P. & Irvine, R. F. Biochem. J. 212, 473–482 (1983).

    Article  CAS  Google Scholar 

  12. Berridge, M. J. Biochem. J. 212, 849–858 (1983).

    Article  CAS  Google Scholar 

  13. Streb, H. & Schulz, I. Am. J. Physiol. (in the press).

  14. Affolter, H. & Sigel, E. Analyt. Biochem. 97, 315–319 (1979).

    Article  CAS  Google Scholar 

  15. Tomlinson, R. V. & Ballou, C. E. J. biol. Chem. 236, 1902–1906 (1961).

    CAS  PubMed  Google Scholar 

  16. Wakasugi, H., Kimura, T., Haase, W., Kribben, A., Kaufmann, R. & Schulz, I. J. Membrane Biol. 65, 205–220 (1982).

    Article  CAS  Google Scholar 

  17. Lucas M., Galvan, A., Solano, P. & Goberna, R. Biochim. biophys. Acta 731, 129–136 (1982).

    Article  Google Scholar 

  18. Downes, C. P., Mussat, M. C. & Michell, R. H. Biochem. J. 203, 169–177 (1982).

    Article  CAS  Google Scholar 

  19. Irvine, R. F., Hemington, N. & Dawson, R. M. C. Biochem. J. 176, 475–484 (1978).

    Article  CAS  Google Scholar 

  20. Irvine, R. F., Letcher, A. J. & Dawson, R. M. C. Biochem. J. 178, 497–500 (1979).

    Article  CAS  Google Scholar 

  21. Irvine, R. F., Letcher, A. J. & Dawson, R. M. C. Biochem. J. 192, 279–283 (1980).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Max-Planck-Institut für Biophysik, Kennedyallee 70, 6000, Frankfurt, (Main) 70, FRG

    H. Streb & I. Schulz

  2. Department of Biochemistry, ARC Institute of Animal Physiology, Babraham, Cambridge, CB2 4AT, UK

    R. F. Irvine

  3. ARC Unit of Insect Neurophysiology and Pharmacology, Department of Zoology, University of Cambridge, Downing Street, Cambridge, CB2 3EJ, UK

    M. J. Berridge

Authors
  1. H. Streb
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. R. F. Irvine
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. M. J. Berridge
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. I. Schulz
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Streb, H., Irvine, R., Berridge, M. et al. Release of Ca2+ from a nonmitochondrial intracellular store in pancreatic acinar cells by inositol-1,4,5-trisphosphate. Nature 306, 67–69 (1983). https://doi.org/10.1038/306067a0

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/306067a0

  • Springer Nature Limited

This article is cited by

Search

Navigation