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Eukaryotic RNA polymerase II binds to nucleosome cores from transcribed genes

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Abstract

Purified RNA polymerase II from calf thymus can bind to about 15% of the nucleosome core particles prepared from mouse myeloma cells, forming a discrete complex having a sedimentation coefficient of 18S. These bound nucleosome cores are heavily enriched in transcribed DNA sequences, are deficient in histones H2A and H2B, and undergo a reversible change in structure on RNA polymerase II binding.

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Authors and Affiliations

  1. MRC Laboratory of Molecular Biology, Hills Road, Cambridge, CB2 2QH, UK

    Bradford W. Baer & Daniela Rhodes

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  1. Bradford W. Baer
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  2. Daniela Rhodes
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Baer, B., Rhodes, D. Eukaryotic RNA polymerase II binds to nucleosome cores from transcribed genes. Nature 301, 482–488 (1983). https://doi.org/10.1038/301482a0

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