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uvr Genes function differently in repair of acetylaminofluorene and aminofluorene DNA adducts

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Abstract

The repair of UV-damaged DNA in Escherichia coli is controlled by the uvr genes (A, B and C)1,2. These genes are also thought to be involved in the repair of chemically damaged DNA3–5, however their specific roles in repairing the multiplicity of different adducts that can be produced by chemical carcinogens are not well understood. We have investigated the repair of DNA damaged by different chemical carcinogens and transfected into E. coli strains with mutations in different uvr genes. The major product of DNA damage by N-acetoxy-2-acetylaminofluorene (NA-AAF) is N-(guanin-C8-yl)-2-acetylaminofluorene (G-C8-AAF)6. Our results suggest that, like UV-induced pyrimidine dimers, the repair of this adduct requires all three uvr genes. However, the repair of DNA damaged by N-hydroxy-aminofluorene (N-OH-AF, the major adduct of which is N-(guanin-C8-yl)-2-aminofluorene, G-C8-AF) seems only to require the uvrC gene.

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Author notes

  1. Moon-shong Tang

    Present address: Science Park—Research Division, The University of Texas System Cancer Center, Smithville, Texas, 78957, USA

Authors and Affiliations

  1. Department of Pathology, Washington University School of Medicine, St Louis, Missouri, 63110, USA

    Moon-shong Tang & Michael W. Lieberman

  2. Department of Chemical Carcinogenesis, Michigan Cancer Foundation, Detroit, Michigan, 48201, USA

    Charles M. King

Authors
  1. Moon-shong Tang
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  2. Michael W. Lieberman
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  3. Charles M. King
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Tang, Ms., Lieberman, M. & King, C. uvr Genes function differently in repair of acetylaminofluorene and aminofluorene DNA adducts. Nature 299, 646–648 (1982). https://doi.org/10.1038/299646a0

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  • DOI: https://doi.org/10.1038/299646a0

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