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De novo methylation and expression of retroviral genomes during mouse embryogenesis

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Abstract

Retrovirus genomes introduced into mouse zygotes by microinjection of cloned DNA, or into morula stage pre-implantation mouse embryos by infection with Moloney murine leukaemia virus (M-MuLV), became de novo methylated and were blocked in expression. No restriction of virus expression and no de novo methylation were observed when post-implantation mouse embryos were infected with virus. Efficient de novo methylation activity may be an important characteristic of gene regulation in early mouse embryos.

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Authors and Affiliations

  1. Heinrich-Pette-Institut für Experimentelle Virologie und Immunologie an der Universität Hamburg, Martinistrasse 52, 2000, Hamburg, 20, FRG

    Detlev Jähner, Heidi Stuhlmann, Colin L. Stewart, Klaus Harbers, Jürgen Löhler, Iva Simon & Rudolf Jaenisch

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  1. Detlev Jähner
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  2. Heidi Stuhlmann
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  3. Colin L. Stewart
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  4. Klaus Harbers
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  5. Jürgen Löhler
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  6. Iva Simon
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  7. Rudolf Jaenisch
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Jähner, D., Stuhlmann, H., Stewart, C. et al. De novo methylation and expression of retroviral genomes during mouse embryogenesis. Nature 298, 623–628 (1982). https://doi.org/10.1038/298623a0

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  • DOI: https://doi.org/10.1038/298623a0

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