Abstract
A transforming gene isolated from T24 human bladder carcinoma cells is closely related to the BALB murine sarcoma virus (MSV) onc gene (v-bas). This transforming gene is localized to a 4.6 kilobase pair (kbp) region and is expressed as a 1.2-kbp polyadenylated transcript, which contains v-bas related sequences. Moreover, antisera known to detect the immunologically related onc gene products of BALB- and Harvey-MSVs recognized elevated levels of a related protein in T24 cells. The normal human homologue of v-bas was found to be indistinguishable from the T24 oncogene by heteroduplex and restriction enzyme analysis. These results imply that rather subtle genetic alterations have led to the activation of the normal human homologue of v-bas as a human transforming gene.
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References
Cold Spring Harb. Symp. quant. Biol. 44, pt 2 (1980).
Frankel, A. E., Gilbert, J. H., Porzig, K. J., Scolnick, E. M. & Aaronson, S. A. J. Virol. 30, 821–827 (1979).
Shibuya, M., Hanafusa, T., Hanafusa, H. & Stephenson, J. R. Proc. natn. Acad. Sci. U.S.A. 77, 6536–6540 (1980).
Andersen, P. R. et al. Cell 26, 129–134 (1981).
Barbacid, M., Breitman, M. L., Lauver, A. L., Long, L. K. & Vogt, P. K. Virology 110, 411–419 (1981).
Beemon, K. Cell 24, 145–153 (1981).
Wang, L. H. et al. J. Virol. 40, 258–267 (1981).
Shih, C., Shilo, B. Z., Goldfarb, M. P., Dannenberg, A. & Weinberg, R. A. Proc. natn. Acad. Sci. U.S.A. 76, 5714–5718 (1979).
Cooper, G. M., Okenquist, S. & Silverman, L. Nature 284, 418–421 (1980).
Cooper, G. M. & Neiman, P. E. Nature 287, 656–659 (1980).
Hopkins, N., Besmer, P., DeLeo, A. B. & Law, L. W. Proc. natn. Acad. Sci. U.S.A. 78, 7555–7559 (1981).
Lane, M. A., Sainten, A. & Cooper, G. M. Proc. natn. Acad. Sci. U.S.A. 78, 5185–5189 (1981).
Shih, C., Padhy, C., Murray, M. & Weinberg, R. A. Nature 290, 261–264 (1981).
Krontikis, T. G. & Cooper, G. M. Proc. natn. Acad. Sci. U.S.A. 78, 1181–1184 (1981).
Murray, M. J. et al. Cell 25, 355–361 (1981).
Perucho, M. et al. Cell 27, 467–476 (1981).
Smith, B. L., Anisowicz, A., Chodosh, L. A. & Sager, R. Proc. natn. Acad. Sci. U.S.A. 79, 1964–1968 (1982).
Lane, M. A., Sainten, A. & Cooper, G. M. Cell 28, 873–880 (1982).
Pulciani, S. et al. Proc. natn. Acad. Sci. U.S.A. 79, 2845–2849 (1982).
Bubenik, J. et al. Int. J. Cancer 11, 765–773 (1973).
Marshall, C. J., Franks, L. M. & Carbonell, A. W. J. natn. Cancer Inst. 58, 1743–1747 (1977).
Goldfarb, M., Shimizu, K., Perucho, M. & Wigler, M. Nature 296, 404–409 (1982).
Shih, C. & Weinberg, R. A. Cell 29, 161–169 (1982).
Lautenberger, J. A., Schulz, R. A., Garon, C. F., Tsichlis, P. N. & Papas, T. S. Proc. natn. Acad. Sci. U.S.A. 78, 1518–1522 (1981).
Souza, L. M., Komaromy, M. C. & Baluda, M. A. Proc. natn. Acad. Sci. U.S.A. 77, 3004–3008 (1980).
Shalloway, D., Zelentz, A. D. & Cooper, G. M. Cell 24, 531–541 (1981).
Srinivasan, A., Reddy, E. P. & Aaronson, S. A. Proc. natn. Acad. Sci. U.S.A. 78, 2077–2081 (1981).
Vande Woude, G. F. et al. Proc. natn. Acad. Sci. U.S.A. 76, 4464–4468 (1979).
Tronick, S. R. et al. Proc. natn. Acad. Sci. U.S.A. 76, 6314–6318 (1979).
Sherr, C. J., Fedele, L. A., Oskarsson, M., Maizel, J. & Vande Woude, G. F. J. Virol. 34, 200–212 (1980).
Robbins, K. C., Devare, S. G. & Aaronson, S. A. Proc. natn. Acad. Sci. U.S.A. 78, 2918–2922 (1981).
Santos, E., Pulciani, S. & Barbacid, M. Fedn Proc. (in the press).
Eva, A. et al., Nature 295, 116–119 (1982).
Lawn, R. M., Fritsch, E. F., Parker, R. C., Blake, G. & Maniatis, T. Cell 15, 1157–1174 (1978).
Shih, T. Y., Weeks, M. O., Young, H. A. & Scolnick, E. M. Virology 96, 64–79 (1979).
Aaronson, S. A. & Todaro, G. J. Virology 36, 254–261 (1968).
Rasheed, S., Nelson-Rees, W. A., Toth, E. M., Arnstein, P. & Gardner, M. B. Cancer 33, 1027–1033 (1974).
Jainchill, J. L., Aaronson, S. A. & Todaro, G. J. J. Virol. 4, 549–553 (1969).
Peters, R. L., Rabstein, L. S., Van Vleck, R., Kelloff, G. J. & Huebner, R. J. J. natn. Cancer Inst. 53, 1725–1731 (1974).
Harvey, J. J. Nature 204, 1104–1107 (1964).
Scher, C. D., Scolnick, E. M. & Siegler, R. Nature 256, 225–228 (1975).
Southern, E. M. J. molec. Biol. 98, 503–517 (1975).
Davis, R. W., Simon, M. & Davidson, N. Meth. Enzym. 21, 413–428 (1971).
Cox, R. A. Meth. Enzym. 12, 120–129 (1967).
Aviv, H. & Leder, P. Proc. natn. Acad. Sci. U.S.A. 69, 1408–1412 (1972).
Bailey, J. M. & Davidson, N. Analyt. Biochem. 70, 75–85 (1976).
Barbacid, M., Lauver, A. L. & Devare, S. J. Virol. 33, 196–207 (1980).
Bonner, W. M. & Laskey, R. A. Eur. J. Biochem. 46, 83–88 (1974).
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Santos, E., Tronick, S., Aaronson, S. et al. T24 human bladder carcinoma oncogene is an activated form of the normal human homologue of BALB- and Harvey-MSV transforming genes. Nature 298, 343–347 (1982). https://doi.org/10.1038/298343a0
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DOI: https://doi.org/10.1038/298343a0
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