Abstract
A monolayer of epithelial cells, endowed with unique secretory properties, lines the human amniochorion1,2. It has been shown that transplantation of these membranes into allogeneic hosts does not result in overt acute graft rejection3–6. We demonstrate here that HLA-A, -B, -C and -DR antigens and β2 microglobulin (β2m) cannot be detected by immunofluorescence on freshly collected or in vitro cultured amniotic epithelial cells, although small quantities of such antigens were detected when more sensitive radiobiological techniques were used. We also show that these cells, cultured in vitro, produce large quantities of enzymes absent in patients with lysosomal storage diseases and that their supernatants can correct glycosaminoglycan accumulation in cultured fibroblasts from patients with Hurler's and Hunter's syndromes.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bourne, G. The Human Amnion and Chorion (Lloyd-Luke, London, 1962).
Hoyes, A. D. Obstet. Gynec. A 4, 1 (1975).
Trelford, J. D. & Trelford-Sander, M. Am. J. Obstet. Gynec. 134, 835 (1977).
Bennett, J. P., Matthews, R. N. & Faulk, W. P. Lancet i, 1153 (1980).
Faulk, W. P. et al. Lancet i, 1156 (1980).
Akle, C., Adinolfi, M., Welsh, K. I., Leibowitz, S. & McColl, I. Lancet ii, 1003, 1005 (1981).
Burgos, H. & Faulk, W. P. Br. J. Obstet. Gynaec. 88, 294 (1981).
Trowsdale, J., Travers, P., Bodmer, W. F. & Patillo, R. A. J. exp. Med. 152, 115 (1980).
Brodsky, F. M., Perham, P. & Bodmer, W. F. Eur. J. Immun. 9, 536 (1979).
Hsi, B.-L., Yeh, G. C. J. & Faulk, W. P. Placenta (in the press).
Fensom, A. H., Benson, P. F., Babarik, A. W., Grant, A. R. & Jacobs, L. Biochem. biophys. Res. Commun. 74, 877 (1977).
Stirling, J. L. et al. Lancet ii, 37 (1979).
Fensom, A. H., Benson, P. F., Grant, A. R. & Jacobs, L. J. inherited metab. Dis. 2, 9 (1979).
Baum, H., Dodgson, K. S. & Spencer, B. Clinica chim. Acta 4, 453 (1959).
Vanier, M. T. Adv. exp. Med. Biol. 101, 731 (1978).
Fratantoni, J. C., Hall, C. W. & Neufeld, E. F. Proc. natn. Acad. Sci. U.S.A. 64, 360 (1969).
Akle, C., Adinolfi, M. & McColl, I. Biol. Res. Pregnancy 2, 23 (1981).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Adinolfi, M., Akle, C., McColl, I. et al. Expression of HLA antigens, β2-microglobulin and enzymes by human amniotic epithelial cells. Nature 295, 325–327 (1982). https://doi.org/10.1038/295325a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/295325a0
- Springer Nature Limited
This article is cited by
-
The developments in amniotic membrane transplantation in glaucoma and vitreoretinal procedures
International Ophthalmology (2023)
-
Inter-placental variability is not a major factor affecting the healing efficiency of amniotic membrane when used for treating chronic non-healing wounds
Cell and Tissue Banking (2023)
-
Human amniotic membrane: an improvement in the treatment of Medication-related osteonecrosis of the jaw (MRONJ)? A case–control study
Cell and Tissue Banking (2022)
-
Amnion Epithelial Cells — a Therapeutic Source
Current Stem Cell Reports (2021)
-
Human Amniotic Epithelial Cells Alleviate a Mouse Model of Parkinson’s Disease Mainly by Neuroprotective, Anti-Oxidative and Anti-Inflammatory Factors
Journal of Neuroimmune Pharmacology (2021)