Abstract
A rise in the concentration of cyclic AMP inhibits secretion in human platelets1–3. In mammalian cells the mechanism of action of cyclic AMP involves activation of a protein kinase and results in protein phosphorylation4. It has previously been shown that myosin light chain kinase purified from smooth muscle is a substrate for the catalytic subunit of cyclic AMP-dependent protein kinase5 and that phosphorylation of smooth muscle myosin kinase results in a decrease in its activity6. Here we present evidence that platelet myosin kinase is a substrate for the catalytic subunit of protein kinase and that phosphorylation of this myosin kinase, isolated from a non-muscle cell, decreases the activity of this enzyme. Dephosphorylation of platelet myosin kinase by a purified phosphatase7 restores its original activity. A decrease in myosin kinase activity in platelets would increase the relative amount of unphosphorylated myosin, which unlike phosphorylated myosin, cannot interact with actin8,9. These findings suggest a mechanism by which cyclic AMP might modulate actin–myosin interaction in platelets and other non-muscle cells.
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References
Haslam, R. J. & Lynham, J. A. Biochem. biophys. Res. Commun. 77, 714–722 (1977).
Haslam, R. J. & Lynham, J. A. Thromb. Res. 12, 619–628 (1978).
Haslam, R. J., Lynham, J. A. & Fox, J. E. B. Biochem. J. 178, 397–407 (1979).
Krebs, E. G. & Beavo, J. A. A. Rev. Biochem. 48, 923–959 (1979).
Adelstein, R. S., Conti, M. A., Hathaway, D. R. & Klee, C. B. J. biol. Chem. 253, 8347–8350 (1978).
Conti, M. A. & Adelstein, R. S. J. biol. Chem. 256, 3178–3182 (1981).
Pato, M. D. & Adelstein, R. S. J. biol. Chem. 255, 6535–6538 (1980).
Adelstein, R. S. & Conti, M. A. Nature 256, 597–598 (1975).
Lebowitz, E. A. & Cooke, R. J. biol. Chem. 253, 5443–5447 (1978).
Hathaway, D. R. & Adelstein, R. S. Proc. natn. Acad. Sci. U.S.A. 76, 1653–1657 (1979).
Dabrowska, R. & Hartshorne, D. J. Biochem. biophys. Res. Commun. 85, 1352–1359 (1978).
Scordilis, S. P. & Adelstein, R. S. J. biol. Chem. 253, 9041–9048 (1978).
Trotter, J. A. & Adelstein, R. S. J. biol. Chem. 254, 8781–8785 (1979).
Yerna, M.-J., Aksoy, M. O., Hartshorne, D. J. & Goldman, R. D. J. Cell Sci. 31, 411–429 (1978).
Scholey, J. M., Taylor, K. A. & Kendrick-Jones, J. Nature 287, 233–235 (1980).
Kerrick, W. G. L., Hoar, P. E., Cassidy, P. S. & Malencik, D. A. Regulation of Muscle Contraction: Excitation–Contraction Coupling (eds Grinnell, A. D. & Brazier, M. A. B.) 227–239 (Academic, New York, 1980).
Daniel, J. L., Molish, I. R., Holmsen, H. & Salganicoff, L. Cold Spring Harb. Conf. Cell Proliferation Vol. 8 (in the press).
Walsh, D. A. et al. J. biol. Chem. 246, 1977–1985 (1971).
Klee, C. B. Biochemistry 16, 1017–1024 (1977).
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Hathaway, D., Eaton, C. & Adelstein, R. Regulation of human platelet myosin light chain kinase by the catalytic subunit of cyclic AMP-dependent protein kinase. Nature 291, 252–254 (1981). https://doi.org/10.1038/291252a0
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DOI: https://doi.org/10.1038/291252a0
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