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Molecular cloning and characterization of cDNA sequences coding for rat relaxin

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Abstract

Using a synthetic oligonucleotide primer, cloned DNA fragments, each containing the entire coding sequence of rat relaxin, have been isolated from a clone ‘bank’ of ovarian mRNA sequences. The nucleotide sequence of these clones demonstrates that relaxin is synthesized as a preprorelaxin molecule with an unexpectedly large connecting peptide of 105 amino acid residues.

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References

  1. Belt, W. D., Anderson, L. L., Cavazos, L. F. & Melampy, R. M. Endocrinology 89, 1–10 (1971).

    Article  CAS  Google Scholar 

  2. Anderson, L. L., Bast, J. D. & Melampy, R. M. J. Endocr. 59, 371–372 (1973).

    Article  CAS  Google Scholar 

  3. O'Byrne, E. et al. J. clin. Endocr. Metab. 47, 1106–1110 (1978).

    Article  CAS  Google Scholar 

  4. Schwabe, C. et al. Recent Prog. Horm. Res. 34, 123–211 (1978).

    CAS  PubMed  Google Scholar 

  5. Porter, D. G. & Downing, S. J. Reprod. Fert. 52, 95–102 (1978).

    Article  CAS  Google Scholar 

  6. James, R., Niall, H., Kwok, S. & Bryant-Greenwood, G. D. Nature 267, 544–546 (1977).

    Article  ADS  CAS  Google Scholar 

  7. Niall, H. D. et al. in Proc. 15th Mid-West Conf. Endocr. Metab., 1980 (ed. Anderson, R.) (Plenum, New York, in the press).

    Google Scholar 

  8. Bradshaw, R. A. Rev. Biochem. 47, 191–216 (1978).

    Article  CAS  Google Scholar 

  9. Tager, H. S. & Steiner, D. F. A. A. Rev. Biochem. 43, 509–538 (1974).

    Article  CAS  Google Scholar 

  10. Gast, M. J., Mercado-Simmen, R., Niall, H. & Boime, I. Ann. N.Y.Acad. Sci. 343, 148–154 (1980).

    Article  ADS  CAS  Google Scholar 

  11. John, M. J., Walsh, J. R., Borgesson, B. W. & Niall, H. D. Endocrinology 108, 726–729 (1981).

    Article  CAS  Google Scholar 

  12. Ullrich, A. et al. Science 196, 1313–1319 (1977).

    Article  ADS  CAS  Google Scholar 

  13. Seeburg, P. H., Shine, J., Martial, J. A., Baxter, J. D. & Goodman, H. M. Nature 270, 486–494 (1977).

    Article  ADS  CAS  Google Scholar 

  14. Noyes, B. E., Mevarech, M., Stein, R. & Agarwal, K. L. Proc. nain. Acad. Sci. U.S.A. 76, 1770–1774 (1979).

    Article  ADS  CAS  Google Scholar 

  15. Szostak, J. W. et al. Meth. Enzym. 68, 419–428 (1979).

    Article  CAS  Google Scholar 

  16. Sherwood, O. D. & Crnekovic, V. E. Endocrinology 104, 893–897 (1979).

    Article  CAS  Google Scholar 

  17. Bantle, J. A., Maxwell, I. H. & Kahn, W. E. Analyt. Biochem 72, 413–427 (1976).

    Article  CAS  Google Scholar 

  18. Roychoudhury, R., Jay, E. & Wu, R. Nucleic Acids Res. 3, 863–877 (1976).

    Article  CAS  Google Scholar 

  19. Chang, A. C. Y. et al. Nature 275, 617–624 (1978).

    Article  ADS  CAS  Google Scholar 

  20. Grunstein, M. & Wallis, J. Meth. Enzym. 68, 379–389 (1979).

    Article  CAS  Google Scholar 

  21. Richards, R. I., Shine, J., Ullrich, A., Wells, J. R. E. & Goodman, H. M. Nucleic Acids Res. 7, 1137–1146 (1979).

    Article  CAS  Google Scholar 

  22. Proudfoot, N. J. & Brownlee, G. G. Nature 263, 211–214 (1976).

    Article  ADS  CAS  Google Scholar 

  23. Hobart, P., Crawford, R., Shen, L., Pictet, R. & Rutter, W. J. Nature 288, 137–141 (1980).

    Article  ADS  CAS  Google Scholar 

  24. Grantham, R., Gautier, C., Gouy, M., Mercier, R. & Pave, A. Nucleic Acids Res. 8, 49–62 (1980).

    Google Scholar 

  25. Steiner, D. F., Quinn, P. S., Chan, S. J., Marsh, J. & Tager, H. S. Ann. N.Y. Acad. Sci. 343, 1–16 (1980).

    Article  ADS  CAS  Google Scholar 

  26. Rinderknecht, E. & Humbel, R. E. FEBS Lett. 89, 283–286 (1978).

    Article  CAS  Google Scholar 

  27. Busse, W. D. & Carpenter, F. H. Biochemistry 15, 1649–1657 (1976).

    Article  CAS  Google Scholar 

  28. Hamilton, J. W. et al. Proc. natn. Acad. Sci. U.S.A. 71, 653–656 (1974).

    Article  ADS  CAS  Google Scholar 

  29. Maxam, A. M. & Gilbert, W. Proc. natn. Acad. Sci. U.S.A. 74, 560–564 (1977).

    Article  ADS  CAS  Google Scholar 

  30. Sanger, F., Nicklen, S. & Coulson, A. R. Proc. natn. Acad. Sci. U.S.A. 74, 5463–5467 (1977).

    Article  ADS  CAS  Google Scholar 

  31. Walsh, J. R. & Niall, H. D. Endocrinology 107, 1258–1260 (1980).

    Article  CAS  Google Scholar 

  32. Sherwood, O. D. Endocrinology 104, 886–892 (1979).

    Article  CAS  Google Scholar 

  33. Kwok, S. C. M., Chamley, W. A. & Bryant-Greenwood, G. D. Biochem. biophys. Res. Commun. 82, 997–1005 (1978).

    Article  CAS  Google Scholar 

  34. Nakanishi, S. et al. Nature 278, 423–434 (1979).

    Article  ADS  CAS  Google Scholar 

  35. Lomedico, P. et al. Cell 18, 545–558 (1978).

    Article  Google Scholar 

  36. Bell, G. I. et al. Nature 284, 26–32 (1980).

    Article  ADS  CAS  Google Scholar 

  37. Perler, F. et al. Cell 20, 555–566 (1980).

    Article  CAS  Google Scholar 

  38. Fiddes, J. C. et al. Proc. natn. Acad. Sci. U.S.A. 76, 4294–4298 (1979).

    Article  ADS  CAS  Google Scholar 

  39. Wallis, M. Nature 284, 512 (1980).

    Article  ADS  CAS  Google Scholar 

  40. Blundell, T. L. & Humbel, R. E. Nature 287, 781–787 (1980).

    Article  ADS  CAS  Google Scholar 

  41. Anderson, M. L., Long, J. A. & Hayashida, T. Biol. Reprod. 13, 499–504 (1975).

    Article  CAS  Google Scholar 

  42. Kendall, J. Z., Plopper, C. G. & Bryant-Greenwood, G. D. Biol. Reprod. 18, 94–98 (1974).

    Article  Google Scholar 

  43. Hudson, P. et al. Endocrinology 108, 353–356 (1981).

    Article  CAS  Google Scholar 

  44. Dubois, M. P. & Dacheux, J. L. Cell Tissue Res. 187, 201–214 (1978),

    Article  CAS  Google Scholar 

  45. Bryant-Greenwood, G. D. in Proc. 15th Mid-West Conf. Endocr. Metab., 1980 (ed. Anderson, R.) (Plenum, New York, in the press).

    Google Scholar 

  46. Seeberg, P. H. et al. Cell 12, 157–165 (1977).

    Article  Google Scholar 

  47. Denhardt, D. T. Biochem. biophys. Res. Commun. 23, 641–646 (1966).

    Article  CAS  Google Scholar 

  48. Sanger, F. & Coulson, A. R. FEBS Lett. 87, 107–110 (1978).

    Article  CAS  Google Scholar 

  49. Taylor, J. M., Illmersee, R. & Summers, J. Biochim. biophys. Acta 442, 324–330 (1976).

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Howard Florey Institute of Experimental Physiology and Medicine, University of Melbourne, Parkville, Victoria, Australia, 3052

    P. Hudson, J. Haley & H. Niall

  2. Department of Genetics, Research School of Biological Sciences, Australian National University, Canberra, Australia, 2601

    M. Cronk & J. Shine

Authors
  1. P. Hudson
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  2. J. Haley
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  3. M. Cronk
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  4. J. Shine
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  5. H. Niall
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Hudson, P., Haley, J., Cronk, M. et al. Molecular cloning and characterization of cDNA sequences coding for rat relaxin. Nature 291, 127–131 (1981). https://doi.org/10.1038/291127a0

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  • DOI: https://doi.org/10.1038/291127a0

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