Abstract
Polyoma virus can develop lytically in differentiated mouse cells, but is unable to express either early or late functions in undifferentiated early embryonic cells1 or in embryonal carcinoma (EC) cells of the mouse. However, the EC cells become susceptible during differentiation and then behave as do mouse somatic cells2. We have recently described the isolation of a new class of polyoma virus mutant (Py EC) able to develop normally in an EC cell line (PCC4-Aza) that is capable of trigerminal differentiation in vivo3 but exhibits a very limited differentiation in vitro4. The mutants do not grow in another EC cell line (F9) that is ‘blocked’—‘nullipotent’ in vivo, but able to differentiate in vitro when grown in the presence of chemical inducers. Compared with their parental strain, all Py EC genomes analysed so far have an extra 20–50 bases in their HpaII-3 restriction fragment5. We now describe polyoma mutants that will grow in EC F9 cells, and the sequence changes in two of them. The region involved in the mutations can be folded into a putative tRNA-like secondary structure which may be involved in the regulation of viral early transcription with respect to the state of differentiation of the host cell.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Swartzendruber, D. E. & Lehman, J. M. J. cell. Physiol. 85, 179–187 (1975).
Boccara, M. & Kelly, F. Annls Microbiol. Inst. Pasteur, Paris A129, 227–238 (1978).
Jakob, H., Boon, T., Gaillard, J., Nicolas, J. F. & Jacob, F. Annls Microbiol. Inst. Pasteur Paris B124, 269–282 (1973).
Shermann, M. L. in Teratomas and Differentiation (eds Shermann, M. C. & Solter, D.) 189–205 (Academic, New York, 1975).
Soeda, E., Arrand, J. R., Smolar, N., Walsh, J. E. & Griffin, B. E. Nature 283, 445–453 (1980).
Seif, R. & Cuzin, F. J. Virol. 24, 721–728 (1977).
Strickland, S. & Mahdavi, V. Cell 15, 393–403 (1978).
Pope, J. M. & Rowe, W. P. J. exp. Med. 120, 121–128 (1964).
Vasseur, M., Kress, C., Montreau, N. & Blangy, D. Proc. natn. Acad. Sci. U.S.A. 77, 1068–1072 (1980).
Arrand, J. R. et al. J. Virol. 33, 606–618 (1980).
Magnusson, G. & Nilson, M. G. J. Virol. 22, 646–653 (1977).
Katinka, M., Yaniv, M., Vasseur, M. & Blangy, D. Cell 20, 393–399 (1980).
Segal, S., Levine, A. J. & Khoury, G. Nature 280, 335–337 (1979).
Inokuchi, H., Dove, W. F. & Freifelder, D. J. molec. Biol. 74, 721–727 (1973).
Liu, L. F., Liu, C. C. & Alberts, B. M. Cell 19, 697–707 (1980).
Hseih, T. & Brutlag, D. Cell 21, 115–125 (1980).
Grosschedl, R. & Birnstiel, M. L. Proc. natn. Acad. Sci. U.S.A. 77, 1432–1436 (1980).
Benoist, C. & Chambon, P. Proc. natn. Acad. Sci. U.S.A. 77, 3865–3869 (1980).
Dandolo, L. et al. (in preparation).
Berkner, K. L. & Folk, W. R. J. biol. Chem. 252, 3176–3184 (1977).
Maxam, A. M. & Gilbert, W. Meth. Enzym. 65, 499–560.
Sanger, F. & Coulson, A. FEBS Lett. 87, 107–110 (1978).
Laskey, R. A. & Mills, A. D. FEBS Lett. 82, 314–318 (1977).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Katinka, M., Vasseurt, M., Montreau, N. et al. Polyoma DNA sequences involved in control of viral gene expression in murine embryonal carcinoma cells. Nature 290, 720–722 (1981). https://doi.org/10.1038/290720a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/290720a0
- Springer Nature Limited
This article is cited by
-
Cell-type specific activation of the polyomavirus F9-1 regulatory region in transgenic mice
Transgenic Research (1996)
-
Determination of the leukaemogenicity of a murine retrovirus by sequences within the long terminal repeat
Nature (1984)
-
Mode of proviral activation of a putative mammary oncogene (int-1) on mouse chromosome 15
Nature (1984)
-
Transcription of DNA injected into Xenopus oocytes is influenced by template topology
Nature (1983)
-
Cell-specific expression controlled by the 5′-flanking region of insulin and chymotrypsin genes
Nature (1983)