Abstract
We have previously1 demonstrated that DNA of mouse fibroblasts transformed by 3-methylcholanthrene (3-MC) induced foci of transformed cells when applied to monolayer cultures of NIH3T3 cells, which indicates that at least a part of this phenotype is encoded in DNA sequences. However, our conclusions were confined to the effects of DNAs of 3-MC-transformed mouse fibroblasts on recipient NIH3T3 cells, also of mouse fibroblast origin. To elucidate this phenomenon further, we have prepared DNAs from a series of mouse and non-mouse tumour lines of non-fibroblastic origin and investigated whether tumour transforming genes can act across tissue and species barriers to transform NIH3T3 cells. We find that DNAs obtained from human, rabbit and mouse bladder carcinoma lines, a lung carcinoma line and rat neuroblastoma and mouse glioma lines, are able to induce transformation of NIH3T3 cells on transfection.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Shih, C., Shilo, B., Goldfarb, M. P., Dannenberg, A. & Weinberg, R. A. Proc. natn. Acad. Sci. U.S.A. 76, 5714 (1979).
Graham, F. L. & van der Eb, A. J. Virol. 52, 456 (1973).
Schubert, D. et al. Nature 249, 224 (1974).
Dawson, C. T., Sundarraj, N. & Pfeiffer, S. E. J. biol. Chem. 252, 2777 (1977).
Summerhayes, I. C., Sun, T. T. & Chen, L. B. J. Cell Biol. (in the press).
Summerhayes, I. C. & Franks, L. M. J. natn. Cancer. Inst. 62, 1017 (1979).
Marshall, C. J., Franks, L. M. & Carbonell, A. W. J. natn. Cancer Inst. 58, 1743 (1977).
Sugiura, K. & Stock, C. C. Cancer Res. 15, 38 (1955).
Wigler, M. et al. Cell 16, 777 (1979).
Rubin, C. M., Houck, C. M., Deininger, P. L., Friedmann, T. & Schmid, C. W. Nature 284, 372 (1980).
Southern, E. M. J. molec. Biol. 98, 503 (1975).
Jelinek, W. R. et al. Proc. natn. Acad. Sci. U.S.A. 77, 1398 (1980).
Tumilowicz, J. J., Nichols, W. W., Cholon, J. J. & Green, A. E. Cancer Res. 30, 2110 (1970).
Klebe, R. J. & Ruddle, F. H. J. Cell Biol. 43, 69 (1969).
Sugiura, K. in Methods of Animal Experimentation Vol. 2 (ed. Gay, W. I.) 171–222, (Academic, New York, 1969).
Rheinwald, J. G. & Beckett, M. A. Cell 22, 629 (1980).
Giard, D. J. et al. J. natn. Cancer Inst. 51, 1417 (1973).
Peterson, W. D. Jr, Stulberg, C. S. & Simpson, W. F. Proc. Soc. exp. Biol. Med. 136, 1187 (1971).
Rigby, C. C. & Franks, L. M. Br. J. Cancer 24, 746 (1970).
McMahon, J. B., Kelleher, P. C. & Smith, C. J. Biochem. biophys. Res. Commun. 76, 1144 (1977).
Yasumura, Y., Tashjian, A. H. & Sato, G. H. Science 154, 1186 (1966).
Handbook on Genetically Standardized Jax Mice (Jackson Memorial Laboratory, Bar Harbor, 1962).
Rigby, P. W. J., Dickmann, M., Rhodes, C. & Berg, P. J. molec. Biol. 113, 237 (1977).
Wahl, G., Stern, M. & Stark, G. Proc. natn. Acad. Sci. U.S.A. 76, 3683 (1979).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Shih, C., Padhy, L., Murray, M. et al. Transforming genes of carcinomas and neuroblastomas introduced into mouse fibroblasts. Nature 290, 261–264 (1981). https://doi.org/10.1038/290261a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/290261a0
- Springer Nature Limited
This article is cited by
-
Peto’s paradox revisited: black box vs mechanistic approaches to understanding the roles of mutations and promoting factors in cancer
European Journal of Epidemiology (2023)
-
Effects of Alkanna bracteosa extract on the expression level of HSP90α and HER2 genes in human gastric cancer cell line
Advances in Traditional Medicine (2023)
-
The path to the clinic: a comprehensive review on direct KRASG12C inhibitors
Journal of Experimental & Clinical Cancer Research (2022)
-
Human cell transformation by combined lineage conversion and oncogene expression
Oncogene (2021)
-
A new territory and its pioneer: opening up a dominant research stream for a translational research area
Scientometrics (2020)