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An immunoglobulin deletion mutant with implications for the heavy-chain switch and RNA splicing

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Abstract

The IF2 immunoglobulin mutant is a DNA deletion of one coding segment and large sections of the flanking intervening sequences. The deletion results in a new splicing pattern and starts in a DNA region containing tandemly repeated sequences which may carry heavy-chain class switch signals.

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References

  1. Adetugbo, K., Milstein, C. & Secher, D. S. Nature 265, 299–304 (1977).

    Article  ADS  CAS  Google Scholar 

  2. Cotton, R. G. H., Secher, D. S. & Milstein, C. Eur. J. Immun. 3, 135–140 (1973).

    Article  CAS  Google Scholar 

  3. Cowan, N. J., Secher, D. S. & Milstein, C. Eur. J. Biochem. 61, 355–368 (1976).

    Article  CAS  Google Scholar 

  4. Dunnick, W., Rabbitts, T. H. & Milstein, C. Nucleic Acids Res. 8, 1475–1484 (1980).

    Article  CAS  Google Scholar 

  5. Potter, M. in Methods in Cancer Research Vol. 2 (ed. Burch, H.) 105–132 (Academic, New York, 1967).

    Google Scholar 

  6. Horibata, K. & Harris, A. W. Expl Cell Res. 60, 61–77 (1970).

    Article  CAS  Google Scholar 

  7. Franklin, E. C. & Frangione, B. in Contemporary Topics in Molecular Immunology (ed. Inman, F. P.) 89–126 (Plenum, New York, 1975).

    Book  Google Scholar 

  8. Franklin, E. C. J. Immun. 121, 2582–2583 (1978).

    CAS  PubMed  Google Scholar 

  9. Sakano, H. et al. Nature 277, 627–633 (1979).

    Article  ADS  CAS  Google Scholar 

  10. Honjo, T. et al. Cell 18, 559–568 (1979).

    Article  CAS  Google Scholar 

  11. Rabbitts, T. H. et al. Nature 283, 351–356 (1980).

    Article  ADS  CAS  Google Scholar 

  12. Blattner, F. R. et al. Science 196, 161–169 (1977).

    Article  ADS  CAS  Google Scholar 

  13. Schreier, P. G. & Cortese, R. J. molec. Biol. 129, 169–172 (1979).

    Article  CAS  Google Scholar 

  14. Sanger, F. et al. J. molec. Biol. (submitted).

  15. Maxam, A. & Gilbert, W. Proc. natn. Acad. Sci. U.S.A. 74, 560–564 (1977).

    Article  ADS  CAS  Google Scholar 

  16. Lerner, M. R. et al. Nature 283, 220–224 (1980).

    Article  ADS  CAS  Google Scholar 

  17. Catterall, J. F. et al. Nature 257, 510–513 (1978).

    Article  ADS  Google Scholar 

  18. Breathnach, R. et al. Proc. natn. Acad. Sci. U.S.A. 75, 4953–4857 (1978).

  19. Frangione, B. & Franklin, E. C. Nature 281, 600–602 (1979).

    Article  ADS  CAS  Google Scholar 

  20. Pernis, B., Forni, L. & Luizzati, A. L. Cold Spring Harb. Symp. quant. Biol. 41, 175–183 (1976).

    Article  Google Scholar 

  21. Wang, A. C. et al. Proc. natn. Acad. Sci. U.S.A. 66, 337–343 (1970).

    Article  ADS  CAS  Google Scholar 

  22. Sledge, C. et al. Proc. natn. Acad. Sci. U.S.A. 73, 923–927 (1976).

    Article  ADS  CAS  Google Scholar 

  23. Gearhart, P. J. Nature 269, 812–813 (1977).

    Article  ADS  CAS  Google Scholar 

  24. Davis, M. M. et al. Nature 283, 733–739 (1980).

    Article  ADS  CAS  Google Scholar 

  25. Bernard, O., Hozumi, N. & Tonegawa, S. Cell 15, 1133–1144 (1978).

    Article  CAS  Google Scholar 

  26. Seidman, J. G. & Leder, P. Nature 276, 790–795 (1978).

    Article  ADS  CAS  Google Scholar 

  27. Sakano, H. et al. Nature 280, 288–294 (1979).

    Article  ADS  CAS  Google Scholar 

  28. Max, E. E., Seidman, J. G. & Leder, P. Proc. natn. Acad. Sci. U.S.A. 76, 3450–4354 (1979).

    Article  ADS  CAS  Google Scholar 

  29. Rabbitts, T. H. & Matthyssens, G. Proc. natn. Acad. Sci. U.S.A. (submitted).

  30. Kataoka, T. et al. Proc. natn. Acad. Sci. U.S.A. 77, 919–923 (1980).

    Article  ADS  CAS  Google Scholar 

  31. Cory, S. & Adams, J. M. Cell 19, 37–52 (1980).

    Article  CAS  Google Scholar 

  32. Coleclough, C., Cooper, D. & Perry, R. P. Proc. natn. Acad. Sci. U.S.A. 77, 1422–1426 (1980).

    Article  ADS  CAS  Google Scholar 

  33. Honjo, T. & Kataoka, T. Proc. natn. Acad. Sci. U.S.A. 75, 2140–2144 (1978).

    Article  ADS  CAS  Google Scholar 

  34. Yamawaki-Kataoka, Y. et al. Nature 283, 786–789 (1980).

    Article  ADS  CAS  Google Scholar 

  35. Milstein, C. et al. in Cellular Selection and Regulation in the Immune Response (ed. Edelman, G. M.) 245–264 (Raven, New York, 1974).

    Google Scholar 

  36. Wang, J. C. in DNA Synthesis, Present and Future (eds Molineux, I. & Kohrijama, M.) 347–366 (Plenum, New York, 1978).

    Book  Google Scholar 

  37. Tinoco, I. et al. Nature new Biol. 246, 40–41 (1973).

    Article  CAS  Google Scholar 

  38. Rabbitts, T. H., Hamlyn, P. H. & Matthyssens, G. & Roe, B. Can. J. Biochem. 58, 76–187 (1980).

    Article  Google Scholar 

  39. Sakano, H., Maki, R., Kurosawa, Y., Roeder, W. & Tonegawa, S. Nature 286, 676–683 (1980).

    Article  ADS  CAS  Google Scholar 

  40. Southern, E. M. J. molec. Biol. 98, 503–517 (1975).

    Article  CAS  Google Scholar 

  41. Wahl, G. M., Stern, M. & Stark, G. R. Proc. natn. Acad. Sci. U.S.A. 76, 3683–3687 (1979).

    Article  ADS  CAS  Google Scholar 

  42. Jeffreys, A. J. & Flavell, R. A. Cell 12, 429–439 (1977).

    Article  CAS  Google Scholar 

  43. Laskey, R. A. & Mills, A. D. FEBS Lett. 82, 314–316 (1979).

    Article  Google Scholar 

  44. Maniatis, T. et al. Cell 15, 687–701 (1978).

    Article  CAS  Google Scholar 

  45. Hohn, B. & Murray, K. Proc. natn. Acad. Sci. U.S.A. 74, 3259–3263 (1977).

    Article  ADS  CAS  Google Scholar 

  46. Blattner, F. R. et al. Science 202, 1279–1280 (1978).

    Article  ADS  CAS  Google Scholar 

  47. Benton, W. D. & Davis, R. W. Science 196, 180–182 (1977).

    Article  ADS  CAS  Google Scholar 

  48. Bolivary, F. et al. Gene 2, 95–113 (1977).

    Article  Google Scholar 

  49. Baralle, F. E., Shoulders, C. C. & Proudfoot, N. J. Cell (in the press).

  50. Messing, J. et al. Proc. natn. Acad. Sci. U.S.A. 74, 3642–3646 (1977).

    Article  ADS  CAS  Google Scholar 

  51. Cowan, N. J., Secher, D. S. & Milstein, C. J. molec. Biol. 90, 691–701 (1974).

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. MRC Laboratory of Molecular Biology, Hills Road, Cambridge, CB2 2QH, UK

    W. Dunnick, T. H. Rabbitts & C. Milstein

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  1. W. Dunnick
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  2. T. H. Rabbitts
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  3. C. Milstein
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Dunnick, W., Rabbitts, T. & Milstein, C. An immunoglobulin deletion mutant with implications for the heavy-chain switch and RNA splicing. Nature 286, 669–675 (1980). https://doi.org/10.1038/286669a0

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  • DOI: https://doi.org/10.1038/286669a0

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