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Is actin in eye lens a possible factor in visual accommodation?

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Abstract

Actin has been purified from various non-muscle cells and characterized by its molecular weight and ability to polymerize into filaments. Although the occurrence of this protein has been postulated in the mammalian eye lens after observation of actin-like filaments in the electron microscope1,2, definite (bio)chemical proof has been provided only recently3. Amino acid analysis, peptide mapping and affinity chromatography revealed the identity of lens actin with the corresponding protein in other tissues. As the filaments could be obtained by co-isolation with highly purified lens plasma membranes, we were interested to know how the actin-containing structures were located in situ. In the experimental approach reported here, the indirect immunofluorescence technique (IFT)4 was applied to unfixed cryostat sections of lens tissue. The distribution of actin in calf, rat and pigeon lens is described, and evidence from this for the role of actin in visual accommodation discussed.

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Authors and Affiliations

  1. Departments of Biochemistry, University of Nijmegen, Geert Grooteplein Noord 21, Nijmegen, The Netherlands

    M. A. Kibbelaar, F. C. S. Ramaekers, P. J. Ringens, A. M. E. Selten-Versteegen & H. Bloemendal

  2. Cytology and Histology, University of Nijmegen, Geert Grooteplein Noord 21, Nijmegen, The Netherlands

    L. G. Poels & P. H. K. Jap

  3. Department of Autoimmune Diseases of the Central Laboratory of the Netherlands Red Cross Blood Transfusion Service, Amsterdam, The Netherlands

    A. L. van Rossum & T. E. W. Feltkamp

Authors
  1. M. A. Kibbelaar
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  2. F. C. S. Ramaekers
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  3. P. J. Ringens
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  4. A. M. E. Selten-Versteegen
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  5. L. G. Poels
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  6. P. H. K. Jap
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  7. A. L. van Rossum
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  8. T. E. W. Feltkamp
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  9. H. Bloemendal
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Kibbelaar, M., Ramaekers, F., Ringens, P. et al. Is actin in eye lens a possible factor in visual accommodation?. Nature 285, 506–508 (1980). https://doi.org/10.1038/285506a0

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  • DOI: https://doi.org/10.1038/285506a0

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