Abstract
Alterations in β-adrenergic receptor number and function and in the hormonal responsiveness of adenylate cyclase have been observed in transformed cells1–3, and tumours4,5. Phorbol myristate acetate (PMA), a potent tumour promoter in mouse skin, induces a dramatic loss of epidermal responsiveness to catecholamines in vivo6,8, although basal levels of cyclic AMP are not affected7–9. In other work we have shown that PMA treatment does not alter the number or affinity of epidermal β-receptors, although accumulation of cyclic AMP in response to isoprenaline injection is sharply inhibited10. Evidence is presented here that PMA exerts this effect by uncoupling epidermal β-receptors from adenylate cyclase.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Sheppard, J. R., Gormus, R. & Moldow, C. F. Nature 269, 693–695 (1977).
Sheppard, J. R. Proc. natn. Acad. Sci. U.S.A. 74, 1091–1094 (1977).
Makarski, J. S. & Niles, R. M. Expl Cell Res. 114, 191–195 (1978).
Allen, D. O., Munshower, J., Morris, H. P. & Weber, G. Cancer Res. 31, 557–560 (1971).
Boyd, H., Louis, C. J. & Martin, T. J. Cancer Res. 34, 1720–1725 (1974).
Grimm, W. & Marks, F. Cancer Res. 34, 3128–3134 (1974).
Mufson, R. A., Simsiman, R. C. & Boutwell, R. K. Cancer Res. 37, 665–669 (1977).
Belman, S., Troll, W. & Garte, S. J. Cancer Res. 38, 2978–2982 (1978).
Garte, S. J. & Belman, S. Biochem. Biophys. Res. Commun. 84, 489–494 (1978).
Belman, S. & Garte, S. J. Cancer Res. 40, 240–244 (1980).
Maguire, M. E., Ross, E. M. & Oilman, A. G. Adv. Cyclic Nudeotide Res. 8, 1–83 (1977).
Lefkowitz, R. J. & Williams, L. T. Adv. Cyclic Nudeotide Res. 9, 1–18 (1978).
Ross, E. M., Maguire, M. E., Surgill, T. W., Biltonen, R. L. & Oilman, A. G. J. biol. Chem. 252, 5761–5775 (1977).
Lefkowitz, R. J., Mukherjee, C., Coverstone, M. & Caron, M. G. Biochem. biophys. Res. Commun. 60, 703–709 (1974).
Limbird, L. E. & Lefkowitz, R. J. Molec. Pharmac. 12, 559–567 (1976).
Puchwein, G., Pfeuffer, T. & Helmreich, E. J. M. J. biol. Chem. 249, 3232–3240 (1974).
Haga, T., Ross, E. M., Anderson, H. J. & Oilman, A. G. Proc. natn. Acad. Sci. U.S.A. 74, 2016–2020 (1977).
Weinstein, I. B., Wigler, M. & Pietropaolo, C. in Origins of Human Cancer Vol. 4 (eds Hiatt, H. H., Watson, J. D. & Winsten, J. A.) 751–771 (Cold Spring Harbor Laboratory, New York, 1977).
Blumberg, P. M., Driedger, P. E. & Rossow, P. W. Nature 264, 446–447 (1976).
Sivak, A., Mossman, B. T. & Van Duuren, B. L. Biochem. biophys. Res. Commun. 46, 605–609 (1972).
Lee, L. S. & Weinstein, I. B. Science 202, 313–315 (1978).
Rimon, G., Hanski, E., Braun, S. & Levitzki, A. Nature 276, 394–396 (1978).
Switzer, B. R. & Summer, G. K. Clin. chim. Acta 32, 203–206 (1971).
Cornish-Bowden, A. & Koshland, D. E. J. molec. Biol. 95, 201–212 (1975).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Garte, S., Belman, S. Tumour promoter uncouples β-adrenergic receptor from adenyl cyclase in mouse epidermis. Nature 284, 171–173 (1980). https://doi.org/10.1038/284171a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/284171a0
- Springer Nature Limited
This article is cited by
-
Protein kinase C-dependent modulation of stimulatory guanine nucleotide binding protein of fetal rat skin keratinocytes
Archives of Dermatological Research (1996)
-
Modulation of muscarinic receptor-mediated adenylate cyclase and phospholipase C responses in rat retina
Cellular and Molecular Neurobiology (1991)
-
Phorbol ester (TPA) reduces prostaglandin E2-stimulated cAMP production by desensitization of prostaglandin E2 receptors in a clonal osteoblast-like cell line, MOB 3-4
Calcified Tissue International (1991)
-
Inhibitory guanine nucleotide binding protein in pig epidermis: regulation of epidermal adenylate cyclase
Archives of Dermatological Research (1990)
-
Alteration of the GTP-dependent inhibitory pathway of rat striatal adenylate cyclase by phorbol esters
Neurochemical Research (1990)