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Influenza antigenic determinants are expressed from haemagglutinin genes cloned in Escherichia coli

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Abstract

A gene sequence for the fowl plague virus (FPV) haemagglutinin molecule has been inserted into a bacterial plasmid such that its transcription is under the control of a promoter derived from the tryptophan operon. Such plasmids direct the synthesis of a protein that reacts specifically with antisera to FPV haemagglutinin. Evidence is also presented that in some cases DNA inserted at the HindIII site of pBR322 is expressed.

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Authors and Affiliations

  1. Searle Research Laboratories, Lane End Road, High Wycombe, Bucks, UK

    J. S. Emtage, W. C. A. Tacon, G. H. Catlin, B. Jenkins, A. G. Porter & N. H. Carey

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  1. J. S. Emtage
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  2. W. C. A. Tacon
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  3. G. H. Catlin
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  4. B. Jenkins
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  5. A. G. Porter
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  6. N. H. Carey
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Emtage, J., Tacon, W., Catlin, G. et al. Influenza antigenic determinants are expressed from haemagglutinin genes cloned in Escherichia coli. Nature 283, 171–174 (1980). https://doi.org/10.1038/283171a0

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  • DOI: https://doi.org/10.1038/283171a0

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