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A ρ-recognition site on phage λ cro-gene mRNA

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Abstract

The synthesis of RNA catalysed by RNA polymerase from Escherichia coli is terminated at specific sites on DNA templates through the action of a multimeric basic protein known as ρ (refs 1, 2). Three lines of evidence suggest that an interactions of ρ with the nascent RNA is important for this termination. First, ρ binds strongly to RNA3; second, ρ expresses an RNA–dependent ATPase activity which is essential for termination4,5; third, RNA polymerase does not terminate RNA synthesis at ρ-dependent sites when the nascent RNA is digested by ribonuclease during transcription2,6. From the fact that certain RNAs, particularly single-stranded, pyrimidine-rich polymers containing at least 10% cytidylate residues, are more effective than other RNAs at promoting ρ-ATPase, it has been proposed7 that ρ recognises specific sites on a nascent RNA for its action. We present evidence here that ρ does bind at a specific region on a mRNA transcribed from bacteriophage λ DNA.

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References

  1. Roberts, J. W. in RNA Polymerase (eds Losick, R. & Chamberlin, M.) 247–271 (Cold Spring Harbor Laboratory, New York, 1976).

    Google Scholar 

  2. Adhya, S. & Gottesman, M. A. Rev. Biochem. 47, 967–996 (1978).

    Article  CAS  Google Scholar 

  3. Richardson, J. P. Cold Spring Harb. Symp. quant. Biol. 35, 127–133 (1970).

    Article  CAS  Google Scholar 

  4. Howard, B. H. & deCrombrugghe, B. J. biol. Chem. 251, 2520–2524 (1976).

    CAS  PubMed  Google Scholar 

  5. Galluppi, G., Lowery, C. & Richardson, J. P. in RNA Polymerase (eds Losick, R. & Chamberlin, M.) 657–665 (Cold Spring Harbor Laboratory, New York, 1976).

    Google Scholar 

  6. Darlix, J.-L. Eur. J. Biochem. 35, 517–526 (1973).

    Article  CAS  Google Scholar 

  7. Richardson, J. P. Proc. FEBS Meet. 11th 43, 153–162 (1978).

    Google Scholar 

  8. Rosenberg, M., Court, D., Shimatake, H., Brady, C. & Wulff, D. L. Nature 272, 414–423 (1978).

    Article  ADS  CAS  Google Scholar 

  9. Roberts, J. W. Nature 224, 1168–1174 (1969).

    Article  ADS  CAS  Google Scholar 

  10. Galp, G. R. & Richardson, J. P. J. molec. Biol. (submitted).

  11. Roberts, T. M., Shimatake, H., Brady, C. & Rosenberg, M. Nature 270, 274–275 (1977).

    Article  ADS  CAS  Google Scholar 

  12. Schwarz, E., Sherer, G., Hoborm, G. & Kössel, H. Nature 272, 410–414 (1978).

    Article  ADS  CAS  Google Scholar 

  13. Blattner, F. R. & Dahlberg, J. R. Nature new Biol. 237, 227–232 (1972).

    Article  CAS  Google Scholar 

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Bektesh, S., Richardson, J. A ρ-recognition site on phage λ cro-gene mRNA. Nature 283, 102–104 (1980). https://doi.org/10.1038/283102a0

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