Skip to main content
SpringerLink
Log in

Single postsynaptic channel currents in tissue cultured muscle

  • Letter
  • Published:

From Nature

View current issue Submit your manuscript

Abstract

Some of the most compelling evidence for the existence of ionic channels in cell membranes comes from direct recording of quantised current jumps generated by the opening and closing of individual channels. Single-channel jumps have been extensively studied for lipid bilayer membranes doped with various channel-forming additives1. Recently agonist-induced single-channel currents were detected in denervated frog muscle by use of extracellular electrodes, which can isolate the current from a small area of membrane2. The current jumps provide a means for the direct test of many of the inferences about ionic channels which have come from electrical noise analysis3,4. In this report we present measurements of single-channel currents induced by the agonist carbamylcholine in tissue-cultured mammalian muscle. These measurements confirm the earlier noise studies on tissue culture preparations5,6. Recordings of single-channel currents induced by the agonist, suberyldicholine, in avian muscle are presented by Nelson and Sachs7.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Ehrenstein, G. & Lecar, H. Q. Rev. Biophys. 10, 1–34 (1977).

    Article  CAS  Google Scholar 

  2. Neher, E. & Sakmann, B. Nature 260, 799–802 (1976).

    Article  ADS  CAS  Google Scholar 

  3. Neher, E. & Stevens, C. F. A. Rev. Biophys. Bioengng 6, 345–381 (1977).

    Article  CAS  Google Scholar 

  4. De Felice, L. J. Int. Rev. Neurobiol. 20, 169–208 (1977).

    Article  CAS  Google Scholar 

  5. Sachs, F. & Lecar, H. Nature new Biol. 246, 214–216 (1973); Biophys. J. 18, 129–144

    Article  CAS  Google Scholar 

  6. Fischbach, G. D. & Lass, Y. J. Physiol., Lond. 280, 515–526 (1978); J. Physiol, Lond. 28, 527–536 (1978).

    Article  CAS  Google Scholar 

  7. Nelson, D. J. & Sachs, F. Nature 282, 861–863 (1979).

    Article  ADS  CAS  Google Scholar 

  8. Neher, E., Sakmann, B. & Steinbach, J. H. Pflugers Arch. ges Physiol. 375, 219–228 (1978).

    Article  CAS  Google Scholar 

  9. Nelson, P. G., Christian, C. N. & Nirenberg, M. Proc. natn. Acad. Sci. U.S.A. 73, 123–127 (1976).

    Article  ADS  CAS  Google Scholar 

  10. Jackson, M. B., Christian, C. N. & Lecar, H. Soc. Neurosci. Abstr. 5, 482 (1979).

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Laboratory of Biophysics, IRP, National Institute of Neurological and Communicative Disorders and Stroke, National Institutes of Health, Bethesda, Maryland, 20205

    Meyer B. Jackson & Harold Lecar

Authors
  1. Meyer B. Jackson
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Harold Lecar
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Jackson, M., Lecar, H. Single postsynaptic channel currents in tissue cultured muscle. Nature 282, 863–864 (1979). https://doi.org/10.1038/282863a0

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/282863a0

  • Springer Nature Limited

This article is cited by

Search

Navigation