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Rat × rat hybrid myelomas and a monoclonal anti-Fd portion of mouse IgG

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Abstract

MONOCLONAL antibodies can be produced by cultures of permanent cell lines derived by fusion of suitable myelomas with spleen cells from immunised animals1. So far, mainly two mouse myelomas, X63-Ag8 and NSI/1-Ag4.1, have been used for this purpose1,2. When cells from immunised mice are used in the fusion, the mouse × mouse hybrid myelomas can be grown as tumours in appropriate mouse strains. We describe here the preparation and use of a new clone of a rat myeloma, which is suitable for the derivation of rat × rat hybrid myelomas producing specific rat antibodies. The spent medium of hybrid myeloma cultures usually contains 1–20 μg ml−1 antibody. The serum and ascites of the tumour-bearing mice, with few exceptions, yield 1–20 mg ml−1 of antibody, which is 1,000 times more concentrated and very convenient for larger preparations.

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  1. MRC Laboratory of Molecular Biology, Hills Road, Cambridge, UK

    G. GALFRÈ, C. MILSTEIN & B. WRIGHT

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  1. G. GALFRÈ
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  2. C. MILSTEIN
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  3. B. WRIGHT
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GALFRÈ, G., MILSTEIN, C. & WRIGHT, B. Rat × rat hybrid myelomas and a monoclonal anti-Fd portion of mouse IgG. Nature 277, 131–133 (1979). https://doi.org/10.1038/277131a0

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