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A ρ-dependent termination site in the gene coding for tyrosine tRNA su3 of Escherichia coli

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Abstract

A set of partially overlapping DNA restriction fragments that support promoter-dependent transcription of the tRNA1Tyr gene of Escherichia coli has been used to study site-specific termination in vitro. Transcription termination occurs at a specific site 224–226 nucleotides beyond the end of the structural gene and is completely dependent on ρ-factor. Certain features of this site suggest differences from other termination sites previously studied. A role for specific sequence recognition is suggested.

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Author information

Author notes

  1. Hans Küpper

    Present address: Institut für Molekulare Genetik, Im Neuenheimer Feld 230, 6900, Heidelburg, Germany

  2. Takao Sekiya

    Present address: National Cancer Center Institute, 5 Chome, Tsukigi Chuo-ku, Tokyo, Japan

Authors and Affiliations

  1. Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts, 02139

    Hans Küpper & Takao Sekiya

  2. Laboratory of Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, 20014

    Martin Rosenberg

  3. Section of Microbiology and Molecular Biology, Division of Biology and Medicine, Brown University, Providence, Rhode Island, 02912

    James Egan & Arthur Landy

Authors
  1. Hans Küpper
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  2. Takao Sekiya
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  3. Martin Rosenberg
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  4. James Egan
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  5. Arthur Landy
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Küpper, H., Sekiya, T., Rosenberg, M. et al. A ρ-dependent termination site in the gene coding for tyrosine tRNA su3 of Escherichia coli. Nature 272, 423–428 (1978). https://doi.org/10.1038/272423a0

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  • DOI: https://doi.org/10.1038/272423a0

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