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Sequence of cro gene of bacteriophage lambda

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Abstract

KNOWLEDGE of the primary sequences of represser proteins and the DNA operators with which they interact is essential for detailed study of the molecular aspects of the control of gene expression. The sequences of two repressers are known: the cI protein of lambda (ref. 1 and R. Sauer and R. Anderegg, personal communication) and the i gene product of the lactose operon of Escherichia coli (refs 2,3 and P. J. Farabaugh, personal communication). Here we report the DNA sequence for the structural gene of a third represser, the Cro protein of lambda. This protein is of special interest both because of its small size (66 amino acids, as compared with the 236 amino acids of the cI protein and the 360 amino acids of the lac i gene), and because genetic evidence suggests that it interacts with the same operator regions as does the cI protein, although the two proteins probably do not recognise exactly the same DNA bases4–7.

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Authors and Affiliations

  1. Department of Biochemistry and Molecular Biology, Harvard University, Cambridge, Massachusetts, 02138

    THOMAS M. ROBERTS

  2. Laboratory of Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, 20014

    HIROYUKI SHIMATAKE, CATHERINE BRADY & MARTIN ROSENBERG

Authors
  1. THOMAS M. ROBERTS
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  2. HIROYUKI SHIMATAKE
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  3. CATHERINE BRADY
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  4. MARTIN ROSENBERG
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ROBERTS, T., SHIMATAKE, H., BRADY, C. et al. Sequence of cro gene of bacteriophage lambda. Nature 270, 274–275 (1977). https://doi.org/10.1038/270274a0

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