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Synthesis of haemoglobin Wayne in erythroid cells

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Abstract

HETEROZYGOTES for haemoglobin (Hb) Wayne possess two minor haemoglobin components that migrate more rapidly than HbA on electrophoresis at pH8.6. Each of the minor haemoglobin components contains an abnormal α chain in which the carboxyl-terminal tripeptide sequence, Lys-Tyr-Arg, has been replaced by an octapeptide1. The slower of the two components, henceforth designated Wayne-Asn, has the following octapeptide sequence: Asn-Thr-Val-Lys-Leu-Glu-Pro-Arg, whereas the faster one (Wayne-Asp) has exactly the same sequence except that asparagine at position 139 is replaced by aspartic acid. (These components, formerly designated1 Wayne-1 and Wayne-2, respectively, have been renamed for clarity.) This is the first variant described in which deamidation of the gene product is believed to occur. The purpose of this study was to demonstrate the proposed deamidation and to explain another interesting feature of the Hb Wayne phenotype in heterozygotes; namely, the presence of the variant haemoglobins in markedly reduced quantities relative to HbA (3% and 4%, respectively, for Hb Wayne-Asn and Hb Wayne-Asp).

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References

  1. Seid-Akhavan, M., Winter, W. P., Abramson, R. K. & Rucknagel, D. L. Proc. natn. Acad. Sci. U.S.A. 73, 882–886 (1976).

    Article  ADS  CAS  Google Scholar 

  2. Huisman, T. H., Martis, E. A. & Dozy, A. J. Lab. clin. Med. 52, 312–327 (1958).

    CAS  PubMed  Google Scholar 

  3. Clegg, J. B., Naughton, M. A. & Weatherall, D. J. J. molec. Biol. 19, 91–108 (1966).

    Article  CAS  Google Scholar 

  4. DeSimone, J., Kleve, L. & Shaeffer, J. J. Lab. clin. Med. 84, 517–524 (1974).

    CAS  PubMed  Google Scholar 

  5. Robinson, A. B. Proc. natn. Acad. Sci. U.S.A. 71, 885–888 (1974).

    Article  ADS  CAS  Google Scholar 

  6. McKerrow, J. H. & Robinson, A. B. Science 183, 85 (1974).

    Article  ADS  CAS  Google Scholar 

  7. Lai, C. Y., Chen, C. & Horecker, B. L. Biochem. biophys. Res. Commun. 40, 461–468 (1970).

    Article  CAS  Google Scholar 

  8. Midelfort, C. F. & Mehler, A. H. Proc. natn. Acad. Sci. U.S.A. 69, 1816–1819 (1972).

    Article  ADS  CAS  Google Scholar 

  9. Moo-Penn, W. J. et al. J. biol. Chem. 251, 7557–7562 (1976).

    CAS  PubMed  Google Scholar 

  10. Charache, S., Fox, J., McCurdy, P., Kazazian, H. & Winslow, R., J. clin. Invest. 59, 652–658 (1977).

    Article  CAS  Google Scholar 

  11. Blackwell, R. O., Wong Hock Boon, C. S. & Weng, M. I. Biochim. biophys. Acta 278, 482–490 (1972).

    Article  CAS  Google Scholar 

  12. Lehmann, H. & Huntsman, R. G. Man's Haemoglobins 2nd edn. 478 (Lippincott. Philadelphia, 1974).

    Google Scholar 

  13. Bornstein, P. Biochemistry 9, 2408–2420 (1970).

    Article  CAS  Google Scholar 

  14. Chalevelakis, G., Clegg, J. B. & Weatherall, D. J. Proc: natn. Acad. Sci.. U.S.A. 72, 3853–3857 (1975).

    ADS  CAS  Google Scholar 

  15. Wood, W. G. & Stamatoyannopoulos, G. J. clin. Invest. 55, 567–578 (1975).

    Article  CAS  Google Scholar 

  16. Clegg, J. B., Weatherall, D. J. & Milner, P. F. Nature 234, 337–340 (1971).

    Article  ADS  CAS  Google Scholar 

  17. Clegg, J. B. & Weatherall, D. J. Ann. N. Y. Acad. Sci. 232, 168–178 (1974).

    Article  ADS  CAS  Google Scholar 

  18. Kan, Y. W., Todd, D. & Dozy, A. M. Br. J. Haematol. 28, 103–107 (1974).

    Article  CAS  Google Scholar 

  19. DeJong, W. W., Meera Khan, P. & Bernini, L. F. Am. J. hum. Genet. 27, 81–90 (1975).

    CAS  Google Scholar 

  20. Clegg, J. B. et al. 251, 245–247 (1974).

  21. Bradley, T. B., Wohl, R. C. & Smith, G. J. clin. Res. 23, 131a (1975).

    Google Scholar 

  22. Rieder, R. F. J. clin. Invest. 51, 364–372 (1972).

    Article  CAS  Google Scholar 

  23. Rieder, R. F. J. clin. Invest. 50, 388–395 (1971).

    Article  CAS  Google Scholar 

  24. Farace, M. G. & Bank, A. Biochim. biophys. Acta 312, 591–597 (1973).

    Article  CAS  Google Scholar 

  25. Rieder, R. F. & James, G. W. Blood 47, 489–494 (1976).

    CAS  PubMed  Google Scholar 

  26. Franklin Bunn, H., Schmidt, G. F., Haney, D. N. & Dluhy, R. G. Proc. natn. Acad. Sci. U.S.A. 72, 3609–3613 (1975).

    Article  ADS  Google Scholar 

  27. Hollan, S. R. et al. Nature 235, 47–50 (1972).

    Article  ADS  CAS  Google Scholar 

  28. Trabuchet, G., Pagnier, J., Benabadji, M. & Labie, D. Hemoglobin 1, 13–25 (1976–77).

    Article  Google Scholar 

  29. Lowry, O. H., Rosebrough, N. J., Farr, A. & Randall, R. S., J. biol. Cliem. 193, 265–275 (1951).

    CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Human Genetics, University of Michigan Medical School, Ann Arbor, Michigan, 48109

    S. M. HANASH, W. P. WINTER & D. L. RUCKNAGEL

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  1. S. M. HANASH
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  2. W. P. WINTER
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  3. D. L. RUCKNAGEL
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HANASH, S., WINTER, W. & RUCKNAGEL, D. Synthesis of haemoglobin Wayne in erythroid cells. Nature 269, 717–719 (1977). https://doi.org/10.1038/269717a0

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