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Nucleotide sequence of bacteriophage φX174 DNA

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Abstract

A DNA sequence for the genome of bacteriophage φX174 of approximately 5,375 nucleotides has been determined using the rapid and simple ‘plus and minus’ method. The sequence identifies many of the features responsible for the production of the proteins of the nine known genes of the organism, including initiation and termination sites for the proteins and RNAs. Two pairs of genes are coded by the same region of DNA using different reading frames.

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Author information

Author notes

  1. G. M. Air

    Present address: John Curtin School of Medical Research, Microbiology Department, Canberra City, ACT, 2601, Australia

  2. N. L. Brown

    Present address: Department of Biochemistry, University of Bristol, Bristol, BS8 1TD, UK

  3. C. A. Hutchison III

    Present address: Department of Bacteriology and Immunology, University of North Carolina, Chapel Hill, North Carolina, 27514

  4. P. M. Slocombe

    Present address: Max-Planck-Institut für Molekulare Genetik, 1, Berlin, 33, FRG

  5. M. Smith

    Present address: Department of Biochemistry, University of British Columbia, Vancouver, BC, Canada, V6T 1W5

Authors and Affiliations

  1. MRC Laboratory of Molecular Biology, Hills Road, Cambridge, CB2 2QH, UK

    F. Sanger, G. M. Air, B. G. Barrell, N. L. Brown, A. R. Coulson, J. C. Fiddes, C. A. Hutchison III, P. M. Slocombe & M. Smith

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  1. F. Sanger
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  2. G. M. Air
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  3. B. G. Barrell
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  4. N. L. Brown
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  5. A. R. Coulson
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  6. J. C. Fiddes
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  7. C. A. Hutchison III
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  8. P. M. Slocombe
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  9. M. Smith
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Sanger, F., Air, G., Barrell, B. et al. Nucleotide sequence of bacteriophage φX174 DNA. Nature 265, 687–695 (1977). https://doi.org/10.1038/265687a0

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  • DOI: https://doi.org/10.1038/265687a0

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