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Direct estimation of frequency of cytotoxic T lymphocytes by a modified plaque assay

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Abstract

THYMUS-DERIVED lymphocytes (T) have been shown to have a major role in cell-mediated immunity against foreign transplants and syngeneic tumours1,2. In vitro, several assays have been developed to measure thymus-dependent cell-mediated immunity, making use of the cytotoxic potential of these effector cells3. Precise estimates of the number of cytotoxic T lymphocytes (CTL), however, are not possible by available methods. We report here a new method to quantify the CTL present in a mixed cell population. The method is based on the ability of CTL to kill specifically adjacent target cells fixed on monolayers and to form zones of lysis or plaques.

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Authors and Affiliations

  1. Department of Microbiology and Immunology, School of Medicine, University of California, Los Angeles, California, 90024

    BENJAMIN BONAVIDA & BARBARA IKEJIRI

  2. Lautenberg Center for General and Tumor Immunology, Hebrew University, Hadassah Medical School, Jerusalem, Israel

    ELI KEDAR

Authors
  1. BENJAMIN BONAVIDA
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  2. BARBARA IKEJIRI
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  3. ELI KEDAR
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BONAVIDA, B., IKEJIRI, B. & KEDAR, E. Direct estimation of frequency of cytotoxic T lymphocytes by a modified plaque assay. Nature 263, 769–771 (1976). https://doi.org/10.1038/263769a0

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  • DOI: https://doi.org/10.1038/263769a0

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