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Alteration in membrane glycoproteins after type-C virus infection of murine fibroblasts

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Abstract

NEW proteins and glycoproteins appear on cell membranes after infection of the cells by type-C viruses1. Some of these new proteins are virus coded and are positioned in the membrane away from the sites of virus budding2. GP70, the major murine glycoprotein has been identified serologically on the membranes of virus-shedding cells3, transformed but non-virus-producing cells4, and on some chemically induced sarcomas5. The internal core protein p30 has been identified as a common antigen on membranes of type-C virus-infected cells of many species6. It has been suggested that this protein, possibly a product of degraded virus, can bind nonspecifically to virus-shedding cells5. Non-virion virus-coded antigens have also been described. The best characterised tumour-specific surface antigen (TSSA) is that found on avian sarcoma cells7. It consists of a major fucose-containing glycoprotein of molecular weight 100,000 and possibly a minor glycoprotein of 32,000. These glycoproteins do not cross react antigenically with the avian virion glycoproteins. A similar antigen, the feline oncornavirus membrane antigen (FOCMA), has been described on virus-infected feline cells8. This antigen has not been well characterised but it is also thought not to be related to the virion proteins. Evidence has been sought largely unsuccessfully for a comparable TSSA in murine model systems9. There have, however, been a few reports of sarcoma specific antigens10. In this paper we describe alterations which occur in membrane glycoproteins after infection of murine fibroblasts by Moloney leukaemia–sarcoma virus (MSV–MLV-M) and by an N-tropic virus isolated from leukaemic AKR mice (MLV–AKR). The technique of lectin chromatography is used to fractionate glycoproteins from other membrane proteins enabling better characterisation of these important surface molecules.

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Authors and Affiliations

  1. Imperial Cancer Research Fund, Lincolns Inn Fields, London, WC2, UK

    JOHN E. SMART

  2. Tumour Immunology Unit, Department of Zoology, University College London, Gower Street, London, WC1E 6BT, UK

    NANCY HOGG

Authors
  1. JOHN E. SMART
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  2. NANCY HOGG
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SMART, J., HOGG, N. Alteration in membrane glycoproteins after type-C virus infection of murine fibroblasts. Nature 261, 314–316 (1976). https://doi.org/10.1038/261314a0

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