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Nuclease that preferentially inactivates DNA containing mismatched bases

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Abstract

Heteroduplex and homoduplex DNA molecules of bacteriophage SPP1 were treated with DNase I, an enzyme of Ustilago maydis implicated in genetic recombination. A transfection assay with Bacillus subtilis demonstrates that the heteroduplexes are preferentially inactivated.

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Author notes

  1. W. K. Holloman

    Present address: Department of Internal Medicine, School of Medicine, Yale University, 333 Cedar Street, New Haven, Connecticut, 06520

Authors and Affiliations

  1. Max-Planck-Institut für Molekulare Genetik, Ihnestrasse 63-73, 1, Berlin, 33, Dahlem, West Germany

    A. Ahmad

  2. National Institute for Medical Research, Mill Hill, London, NW7 1AA, UK

    R. Holliday

Authors
  1. A. Ahmad
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  2. W. K. Holloman
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  3. R. Holliday
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Ahmad, A., Holloman, W. & Holliday, R. Nuclease that preferentially inactivates DNA containing mismatched bases. Nature 258, 54–56 (1975). https://doi.org/10.1038/258054a0

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  • DOI: https://doi.org/10.1038/258054a0

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