Abstract
mRNAs of many eukaryotic cells and viruses contain blocked, methylated 5′-terminal structures of the type, m7GpppN (ref. 1). This ‘cap’ structure, and in particular the 5′-terminal 7-methylguanosine, is required for efficient translation in vitro of globin, reovirus and vesicular stomatitis virus mRNAs2,3. The genome subunits of RNA tumour viruses probably function as viral mRNA since RNA of the same base sequence is present in virus-specific polysomes of infected cells4,5 and virion genome RNA can be translated in vitro in a eukaryotic system6. Consistent with this possibility, we have found a blocked, methylated 5′-terminal structure—m7GpppGmpCp—in the genome RNA of avian sarcoma virus (ASV).
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Nature, 255, 9 (1975).
Both, G. W., Banerjee, A. K., and Shatkin, A. J., Proc. natn. Acad. Sci. U.S.A., 72, 1189–1193 (1975).
Muthukrishnan, S., Both, G. W., Furuichi, Y., and Shatkin, A. J., Nature, 255, 33–37 (1975).
Schincariol, A. L., and Joklik, W. K., Virology, 56, 532–548 (1973).
Fan, H., and Baltimore, D., J. molec. Biol., 80, 93–117 (1973).
Von der Helm, K., and Duesberg, P. H., Proc. natn. Acad. Sci. U.S.A., 72, 614–618 (1975).
Quintrell, N., Varmus, H. E., Bishop, J. M., Nicholson, M.D., and McAllister, R. M., Virology, 58, 568–575 (1974).
Duesberg, P. H., and Vogt, P. K., J. Virol., 12, 594–599 (1974).
Rosenberg, M., Nucleic Acid Res., 1, 653–671 (1974).
Furuichi, Y., Morgan, M., Muthukrishan, S., and Shatkin, A. J., Proc. natn. Acad. Sci. U.S.A., 72, 362–366 (1975).
Keith, J., Gleason, M., and Fraenkel-Conrat, H., Proc. natn. Acad. Sci. U.S.A., 71, 4371–4375 (1974).
Fujimoto, M., Kuninaka, A., and Yoshino, H., Agric. biol. Chem., 38, 1551–1561 (1974).
Desrosiers, R., Friderici, K., and Rottman, F., Proc. natn. Acad. Sci. U.S.A., 71, 3971–3975 (1974).
Lavi, S., and Shatkin, A. J., Proc. natn. Acad. Sci. U.S.A., 72, 2012–2016 (1975).
Wei, C. -M., Gershowitz, A., and Moss, B., Cell, 4, 379–386 (1975).
Perry, R. P., Kelley, D. E., Friderici, K., and Rottman, F., Cell, 4, 387–394 (1975).
Adams, J. M., and Cory, S., Nature, 255, 28–33 (1975).
Furuichi, Y., et al., Proc. natn. Acad. Sci. U.S.A., 72, 1904–1908 (1975).
Nishimura, S., Progr. Nucleic Acid Res. molec. Biol., 12, 49–85 (1972).
Faras, A. J., et al., J. Virol., 13, 1134–1142 (1974).
Rottman, F., Shatkin, A. J., and Perry, R. P., Cell, 3, 197–199 (1974).
Furuichi, Y., Nucleic Acid Res., 1, 809–822 (1974).
Shatkin, A. J., Proc. natn. Acad. Sci. U.S.A., 71, 3204–3207 (1974).
Wei, C. -M., and Moss, B., Proc. natn. Acad. Sci. U.S.A., 71, 3014–3018 (1974).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
FURUICHI, Y., SHATKIN, A., STAVNEZER, E. et al. Blocked, methylated 5′-terminal sequence in avian sarcoma virus RNA. Nature 257, 618–620 (1975). https://doi.org/10.1038/257618a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/257618a0
- Springer Nature Limited
This article is cited by
-
Momordica charantia phytoconstituents can inhibit human T-lymphotropic virus type-1 (HTLV-1) infectivity in vitro and in vivo
Journal of NeuroVirology (2023)
-
N6-methyladenosine modification enables viral RNA to escape recognition by RNA sensor RIG-I
Nature Microbiology (2020)
-
Viral N6-methyladenosine upregulates replication and pathogenesis of human respiratory syncytial virus
Nature Communications (2019)
-
Post-transcriptional gene regulation by mRNA modifications
Nature Reviews Molecular Cell Biology (2017)
-
Epitranscriptome: m6A and its function in stem cell biology
Genes & Genomics (2017)