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Isolation of histone genes from unfractionated sea urchin DNA by subculture cloning in E. coli

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Abstract

Histone genes have been cloned selectively in Escherichia coli directly from unfractionated sea urchin DNA using labelled mRNA probe. The cloning method used is potentially applicable for isolating any genetic sequence for which a probe is available. The several eukaryotic gene fragments introduced into bacteria by this method collectively contain all or nearly all of the DNA sequences represented in histone mRNA.

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Author notes

  1. Laurence H. Kedes: Also at Veterans Administration Hospital, Palo Alto, California 94304.

  2. David Houseman: Ontario Cancer Institute, Toronto, Canada M4H1A8.

Authors and Affiliations

  1. Departments of Medicine, Stanford University School of Medicine, Stanford, California, 94305

    Laurence H. Kedes, Annie C. Y. Chang, David Houseman & Stanley N. Cohen

Authors
  1. Laurence H. Kedes
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  2. Annie C. Y. Chang
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  3. David Houseman
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  4. Stanley N. Cohen
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Kedes, L., Chang, A., Houseman, D. et al. Isolation of histone genes from unfractionated sea urchin DNA by subculture cloning in E. coli. Nature 255, 533–538 (1975). https://doi.org/10.1038/255533a0

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  • DOI: https://doi.org/10.1038/255533a0

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