Abstract
TREATMENT of a variety of cell types with concanavalin A (con A) leads to the aggregation of the lectin into one region or pole of the cell, forming a so-called cap. Not all cells cap (erythrocytes and normal cultured fibroblasts do not). Cells which do cap can be distinguished as (a) lymphocytes1 and rabbit exudate polymorphonuclear leukocytes (PMN) (our data) that cap directly when incubated with low concentrations of con A at 37° C, and (b) cells exemplified by SV40-transformed fibroblasts2 and lymphocytes incubated with high (more than 5 µg ml−1) concentrations of con A1 that cap only after incubation with colchicine which disrupts microtubules (MT). These differences between cell types are not understood.
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OLIVER, J., ZURIER, R. & BERLIN, R. Concanavalin A cap formation on polymorphonuclear leukocytes of normal and beige (Chediak-Higashi) mice. Nature 253, 471–473 (1975). https://doi.org/10.1038/253471a0
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DOI: https://doi.org/10.1038/253471a0
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