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Immunological distinction between the possible origins of enzymatic activity in a polypeptide fragment of staphylococcal nuclease

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Abstract

WE have used an immunological approach to demonstrate that the residual enzymatic activity in a large polypeptide fragment of staphylococcal nuclease is an intrinsic property of the fragment and not an artefact due to contamination with intact enzyme. These results are consistent with present concepts of the folding of polypeptide fragments to form structures similar to the native protein and may offer a method for the investigation of other macromolecular systems.

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Authors and Affiliations

  1. Immunology Branch, National Cancer Institute and Laboratory of Chemical Biology, National Institute of Arthritis, Metabolism and Digestive Diseases, National Institutes of Health, Bethesda, Maryland, 20014

    DAVID H. SACHS, ALAN N. SCHECHTER, ANN EASTLAKE & CHRISTIAN B. ANFINSEN

Authors
  1. DAVID H. SACHS
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  2. ALAN N. SCHECHTER
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  3. ANN EASTLAKE
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  4. CHRISTIAN B. ANFINSEN
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SACHS, D., SCHECHTER, A., EASTLAKE, A. et al. Immunological distinction between the possible origins of enzymatic activity in a polypeptide fragment of staphylococcal nuclease. Nature 251, 242–244 (1974). https://doi.org/10.1038/251242a0

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  • DOI: https://doi.org/10.1038/251242a0

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