Abstract
THE assembly of contractile and calcium-related regulatory proteins into functioning myofilament arrays within muscle cells and the genetic control of muscle differentiation are still largely unknown processes1–2. Here we present evidence that a particular gene may regulate such lattice assembly in the body wall muscle cells of the nematode Caenorhabditis elegans. A temperature-sensitive mutation in this gene can prevent the appearance of normal myofilament lattices in this muscle without affecting the amounts of major contractile proteins present. The organised or defective adult structures once formed in mutant muscle are stable to changes in temperature.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Etlinger, J. D., and Fischman, D. A., Cold Spring Harb. Symp. quant. Biol., 37, 511–522 (1972).
Yaffe, D., and Dym, H., Cold Spring Harb. Symp. quant. Biol., 37, 543–547 (1972).
Holtzer, H., Sanger, J. W., Ishikawa, H., and Strahs, K., Cold Spring Harb. Symp. quant. Biol., 37, 549–566 (1972).
Brenner, S., Br. Med. Bull., 29, 269–271 (1973).
Brenner, S., Genetics, 77, 71–94 (1974).
Suzuki, D. T., Science, 170, 695–705 (1970).
Laemmli, U. K., and Favre, M., J. molec. Biol., 80, 575–599 (1973).
Hirumi, H., Ruski, D. J., and Jones, N. O., J. ultrastruct. Res., 34, 517–543 (1971).
Epstein, H. F., Waterston, R. H., and Brenner, S., J. molec. Biol. (in the press).
Rosenbluth, J., J. Cell Biol., 25, 495–515 (1965).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
EPSTEIN, H., THOMSON, J. Temperature-sensitive mutation affecting myofilament assembly in Caenorhabditis elegans. Nature 250, 579–580 (1974). https://doi.org/10.1038/250579a0
Received:
Revised:
Published:
Issue Date:
DOI: https://doi.org/10.1038/250579a0
- Springer Nature Limited
This article is cited by
-
Disruption of Drosophila larval muscle structure and function by UNC45 knockdown
BMC Molecular and Cell Biology (2021)
-
Molecular cloning of myosin chaperone uncoordinate-45B (UNC-45B) and its heat stress response in the muscle of Pacific bluefin tuna Thunnus orientalis
Fisheries Science (2021)
-
Molecular features of the UNC-45 chaperone critical for binding and folding muscle myosin
Nature Communications (2019)
-
UFD-2 is an adaptor-assisted E3 ligase targeting unfolded proteins
Nature Communications (2018)
-
Muscle contraction phenotypic analysis enabled by optogenetics reveals functional relationships of sarcomere components in Caenorhabditis elegans
Scientific Reports (2016)