Abstract
GENETIC analysis of i gene mutants which had lost the capacity to bind operator but not inducer suggested that lac repressor binds with its amino terminus to lac operator1. Here we will show that the carboxy terminus of lac repressor is dispensable: We found fusions between the i (lac repressor) and z (β-galactosidase) genes (Fig. 1) which produce fully active lac repressor covalently linked to active β-galactosidase.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Adler, K., et al., Nature, 237, 322–327 (1972).
Müller-Hill, B., Crapo, L., and Gilbert, W., Proc. natn. Acad. Sci U.S.A., 59, 1259–1264 (1968).
Jacob, F., and Monod, J., J. molec. Biol., 3, 318–356 (1961).
Pfahl, M. L., Genetics, 72, 393–410 (1972).
Zipser, D., Zabell, S., Rothman, J., Grodzicker, T., Wenk, M., and Novitski, M., J. molec. Biol., 49, 251–254 (1970).
Miller, J. H., Reznikoff, W. S., Silverstone, A. E., Ippen, K., Signer, E. R., and Beckwith, J. R., J. Bact., 104, 1273–1279 (1970).
Gilbert, W., and Müller-Hill, B., Proc. natn. Acad. Sci. USA., 56, 1891–1898 (1966).
Platt, T., in Experiments in Molecular Genetics (edit. by Miller, J. H.), 403. (Cold Spring Harbor Laboratory, New York, 1972).
Fowler, A. V., and Zabin, I., J. biol. Chem., 245, 5032–5041 (1970).
Beyreuther, K., Adler, K., Geisler, N., and Klemm, A., Proc. natn. Acad. Sci. U.S.A., 70, 3576–3580 (1973).
Weiner, A. M., Platt, T., and Weber, K., J. biol. Chem., 247, 3242–3251 (1972).
Platt, T., Files, J. G., and Weber, K., J. biol. Chem., 248, 110–121 (1973).
Weber, K., and Osborn, M., J. biol. Chem., 244, 4406–4412 (1969).
Riggs, A. D., Suzuki, H., and Bourgeois, S., J. molec. Biol., 48, 67–83 (1970).
Bray, G. A., Analyt. Biochem., 1, 279–285 (1960).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
MüLLER-HILL, B., KANIA, J. Lac repressor can be fused to β-galactosidase. Nature 249, 561–563 (1974). https://doi.org/10.1038/249561a0
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1038/249561a0
- Springer Nature Limited
This article is cited by
-
Antibiotic-induced DNA damage results in a controlled loss of pH homeostasis and genome instability
Scientific Reports (2020)
-
Crosstalk-free colloidosomes for high throughput single-molecule protein analysis
Science China Chemistry (2020)
-
Genome sequences of two closely related strains of Escherichia coli K-12 GM4792
Standards in Genomic Sciences (2015)
-
Efficient secretion of the herpes simplex virus tegument protein VP22 from living mammalian cells
Archives of Virology (2008)
-
The isolated N-terminal DNA binding domain of thec repressor of bacteriophage16-3 is functional in DNA binding in vivo and in vitro
Molecular and General Genetics MGG (1991)