Abstract
CELLS from nine consecutive patients with chronic myelogenous leukaemia (CML) have been analysed with quinacrine fluorescence and various Giemsa staining techniques. The Philadelphia (Ph1) chromosome in all nine patients represents a deletion of the long arm of chromosome 22 (22q−)1,2. An unsuspected abnormality in all cells from the nine patients has been detected with these new staining techniques. It consists of the addition of dully fluorescing material to the end of the long arm of one chromosome 9 (9q+). In Giemsa-stained preparations, this material appears as an additional faint terminal band in one chromosome 9. The amount of additional material is approximately equal to the amount missing from the Ph1 (22q−) chromosome, suggesting that there may be a hitherto undetected translocation between the long arm of 22 and the long arm of 9, producing the 9q+ chromosome.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Chicago Conference. Standardization in human cytogenetics. The National Foundation—March of Dimes (1966).
Paris Conference. Standardization in human cytogenetics. The National Foundation—March of Dimes (1972).
Rowley, J. D., Blaisdell, R. K., and Jacobson, L. O., Blood, 27, 782 (1966).
Rowley, J. D., Potter, D., and Mikuta, J., Stain Tech., 46, 97 (1971).
Pearson, P. L., Bobrow, M., and Vosa, C. G., Nature, 226, 78 (1970).
Sumner, A. T., Evans, H. J., and Buckland, R. A., Nature New Biology, 232, 31 (1971).
Rowley, J. D., and Bodmer, W. F., Nature, 231, 503 (1971).
Caspersson, T., Gahrton, G., Lindsten, J., and Zech, L., Exp. Cell Res., 63, 238 (1970).
O'Riordan, M. L., Robinson, J. A., Buckton, K. E., and Evans, H. J., Nature, 230, 167 (1971).
Rudkin, G. T., and Hungerford, D. A., Science, NY, 144, 1229 (1964).
Whang-Peng, J., Canellos, G. P., Carbone, P. P., and Tjio, J. H., Blood, 32, 755 (1968).
Ezdinli, E. Z., Sokal, J. E., Crosswhite, B. S., and Sandberg, A. A., Ann. Int. Med., 72, 175 (1970).
Lobb, D. S., Reeves, B. R., and Lawler, S. D., Lancet, i 849 (1972).
Rowley, J. D., Ann. Génét. (in the press).
Manolov, G., and Manolova, Y., Hereditas, 69, 300 (1971).
Mark, J., Levan, G., and Mitelman, F., Hereditas, 71, 163 (1972).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
ROWLEY, J. A New Consistent Chromosomal Abnormality in Chronic Myelogenous Leukaemia identified by Quinacrine Fluorescence and Giemsa Staining. Nature 243, 290–293 (1973). https://doi.org/10.1038/243290a0
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1038/243290a0
- Springer Nature Limited
This article is cited by
-
The reckoning of chromosomal instability: past, present, future
Chromosome Research (2024)
-
Leukaemia exposure alters the transcriptional profile and function of BCR::ABL1 negative macrophages in the bone marrow niche
Nature Communications (2024)
-
The prognostic and therapeutic potential of HO-1 in leukemia and MDS
Cell Communication and Signaling (2023)
-
FISH-negative BCR::ABL1-positive e19a2 chronic myeloid leukaemia: the most cryptic of insertions
BMC Medical Genomics (2023)
-
Effectiveness of digital care platform CMyLife for patients with chronic myeloid leukemia: results of a patient-preference trial
BMC Health Services Research (2023)