Skip to main content
SpringerLink
Log in

Cyclic AMP Released by Rat Testis during Gonadotrophin Stimulation in vitro

  • Letter
  • Published:

From Nature

View current issue Submit your manuscript

Abstract

THE stimulation of testosterone secretion from testis interstitial cells by luteinizing hormone (LH) and human chorionic gonadotrophin (hCG) is believed to depend on cyclic AMP formation, as is the case with other steroidogenic tissues1. Addition of cyclic AMP to slices of rat2 and rabbit testis3 stimulates testosterone production, and LH increases adenylate cyclase activity in the testis of dog4 and rat5. We have observed that the isolated rat testis is extremely sensitive to LH and hCG stimulation in vitro, responding to low levels of hCG (1 ng/ml.) with maximum release of testosterone into the incubation medium6. In contrast, the teased rat testis responds poorly to gonadotrophic stimulation, and adenylate cyclase in testis homogenates is relatively insensitive to large doses of LH or hCG7.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Robinson, G. A., Butcher, R. W., and Sutherland, E. W., Ann. Rev. Biochem., 37, 149 (1968).

    Article  Google Scholar 

  2. Sandler, R., and Hall, P. F., Endocrinology, 79, 647 (1966).

    Article  CAS  Google Scholar 

  3. Eik-Nes, K. B., Recent Prog. Hormone Res., 25, 517 (1971).

    Google Scholar 

  4. Murad, F., Strauch, B. S., and Vaughan, M., Biochim. Biophys. Acta, 177, 591 (1969).

    Article  CAS  Google Scholar 

  5. Kuehl, F. A., Panatelli, D. J., Tarnoff, J., and Humes, J. L., Biology of Reproduction, 2, 154 (1970).

    Article  CAS  Google Scholar 

  6. Dufau, M. L., Catt, K. J., and Tsuruhara, T., Endocrinology, 90, 178 (1972).

    Article  Google Scholar 

  7. Dufau, M. L., Catt, K. J., and Tsuruhara, T., Biochim. Biophys. Acta, 252, 574 (1971).

    Article  CAS  Google Scholar 

  8. Kuo, J. F., and De Renzo, E. C., J. Biol. Chem., 244, 2252 (1969).

    CAS  PubMed  Google Scholar 

  9. Steiner, A. L., Kipnis, D. M., Utiger, R., and Parker, C., Proc. US Nat. Acad. Sci., 64, 367 (1969).

    Article  ADS  CAS  Google Scholar 

  10. Henion, W. F., Sutherland, E. W., and Pasternak, T., Biochim. Biophys. Acta, 148, 106 (1967).

    Article  CAS  Google Scholar 

  11. Broadus, A. E., Hardman, J. G., Kaminsky, N. I., Ball, J. H., Sutherland, E. W., and Liddle, G. W., Ann. New York Acad. Sci., 185, 50 (1971).

    Article  ADS  CAS  Google Scholar 

  12. Murad, F., Manganiello, V., and Vaughan, M., J. Biol. Chem., 245, 3352 (1969).

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Reproduction Research Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland, 20014

    K. J. CATT, K. WATANABE & M. L. DUFAU

Authors
  1. K. J. CATT
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. K. WATANABE
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. M. L. DUFAU
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

CATT, K., WATANABE, K. & DUFAU, M. Cyclic AMP Released by Rat Testis during Gonadotrophin Stimulation in vitro. Nature 239, 280–281 (1972). https://doi.org/10.1038/239280a0

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1038/239280a0

  • Springer Nature Limited

This article is cited by

Search

Navigation