Abstract
Homogeneous preparations of initiation factor F3 have both RNA binding and ribosome dissociation activities. But although DF activity could entirely account for the role of F3 in the ribosome cycle this factor has an additional function in formation of the initiation complex that may be related to its RNA binding properties.
Similar content being viewed by others
References
Miller, M. J., Zasloff, M., and Ochoa, S., FEBS Lett., 3, 50 (1969).
Parenti-Rosina, R., Eisenstadt, A., and Eisenstadt, J. M., Nature, 221, 363 (1969).
Iwasaki, K., Sabol, S., Wahba, A. J., and Ochoa, S., Arch. Biochem. Biophys., 125, 542 (1968).
Revel, M., Herzberg, M., Becarevic, A., and Gros, F., J. Mol. Biol., 33, 231 (1968).
Mazumder, R., Chae, Y-B., and Ochoa, S., Proc. US Nat. Acad. Sci., 63, 98 (1969).
Chae, Y-B., Mazumder, R., and Ochoa, S., Proc. US Nat. Acad. Sci., 63, 828 (1969).
Kolakofsky, D., Dewey, K. F., Hershey, J. W. B., and Thach, R. E., Proc. US Nat. Acad. Sci., 61, 1066 (1968).
Lelong, J. C., Grunberg-Manago, M., Dondon, J., Gros, D., and Gros, F., Nature, 226, 505 (1970).
Chae, Y-B., Mazumder, R., and Ochoa, S., Pros. US Nat. Acad. Sci., 62, 1181 (1969).
Hershey, J. W. B., Dewey, K. F., and Thach, R. E., Nature, 222, 944 (1969).
Subramanian, A. R., Davis, B. D., and Beller, R. J., Cold Spring Harbor Symp. Quant. Biol., 34, 223 (1969).
Nomura, M., and Lowry, C. V., Proc. US Nat. Acad. Sci., 58, 946 (1967).
Schlessinger, D., Mangiarotti, G., and Apirion, D., Proc. US Nat. Acad. Sci., 58, 1782 (1967).
Kaempfer, R., Proc. US Nat. Acad. Sci., 61, 106 (1968).
Lowry, O. H., Rosebrough, N. J., Farr, A. L., and Randall, R. J., J. Biol. Chem., 193, 265 (1951).
Weber, K., and Osborn, M., J. Biol. Chem., 244, 4406 (1969).
Cannata, J. J. B., Focesi, jun., A., Mazumder, R., Warner, R. C., and Ochoa, S., J. Biol. Chem., 240, 3249 (1965).
Shapiro, A. L., Viñuela, E., and Maizell, J. V., Biochem. Biophys. Res. Commun., 28, 815 (1967).
Ellman, G. L., Arch. Biochem. Biophys., 82, 70 (1959).
Brown, J. C., and Doty, P., Biochem. Biophys. Res. Commun., 30, 284 (1968).
Kohler, R. W., Ron, E. Z., and Davis, B. D., J. Mol. Biol., 36, 71 (1968).
Subramanian, A. R., Ron, E. Z., and Davis, B. D., Proc. US Nat. Acad. Sci., 61, 761 (1968).
Miller, M. J., thesis, New York University (1969).
Gonzalez, N. S., Bade, E. G., and Algranati, I. D., FEBS Lett., 4, 331 (1969).
Albrecht, J., Stap, F., Voorma, H. O., van Knippenberg, P. H., and Bosch, L., FEBS Lett., 6, 297 (1970); Albrecht, J., thesis, Univ. Leiden (1970).
Miall, S. H., Kato, T., and Tamaoki, T., Nature, 226, 1050 (1970).
McLaughlin, C. S., Dondon, J., Grunberg-Manago, M., Michelson, A. M., and Saunders, G., J. Mol. Biol., 32, 521 (1968).
Kondo, M., Eggerston, G., Eisenstadt, J., and Lengyel, F., Nature, 220, 368 (1968).
Ghosh, H. P., and Khorana, H. G., Proc. US Nat. Acad. Sci., 58, 2455 (1967).
Nirenberg, M. W., and Leder, P., Science, 145, 1399 (1964).
Algranati, I. D., Gonzalez, N. S., and Bade, E. G., Proc. US Nat. Acad. Sci, 62, 574 (1969).
Friedman, H., Lu, P., and Rich, A., Nature, 223, 909 (1969).
Revel, M., Lelong, J. C., Brawerman, G., and Gros, F., Nature, 219, 1016 (1968).
Greenshpan, H., and Revel, M., Nature, 224, 331 (1969).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
SABOL, S., SILLERO, M., IWASAKI, K. et al. Purification and Properties of Initiation Factor F3. Nature 228, 1269–1273 (1970). https://doi.org/10.1038/2281269a0
Received:
Issue Date:
DOI: https://doi.org/10.1038/2281269a0
- Springer Nature Limited
This article is cited by
-
Specific binding site of E. coli initiation factor 3 (IF3) at a 3′-terminal region of MS2 RNA
Nature (1977)
-
Initiation of protein synthesis
Die Naturwissenschaften (1976)
-
The role of IF-3 in the translation of T7-and ϕ80trp messenger RNA
Molecular and General Genetics MGG (1975)