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Fast Spectrophotometric Measurement of H+ Changes in Chromatium Chromatophores activated by a Liquid Dye Laser

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Abstract

The fast changes in hydrogen ion concentration which follow photo-activation of the bacteriochlorophyll complex of Chromatium have been measured by observing absorbance changes of the pH indicator bromcresol purple using a ruby-pumped dye laser. This acts as an optical frequency converter with an output pulse corresponding to an absorption peak of the chromatophore. The changes in H+ binding represent a sort of “membrane Bohr effect”.

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Authors and Affiliations

  1. Johnson Research Foundation, School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania

    B. CHANCE, J. A. McCRAY & J. BUNKENBURG

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  1. B. CHANCE
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  2. J. A. McCRAY
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  3. J. BUNKENBURG
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CHANCE, B., McCRAY, J. & BUNKENBURG, J. Fast Spectrophotometric Measurement of H+ Changes in Chromatium Chromatophores activated by a Liquid Dye Laser. Nature 225, 705–708 (1970). https://doi.org/10.1038/225705a0

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