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Control of Degradation and Synthesis of Induced Tyrosine Aminotransferase studied in Hepatoma Cells in Culture

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Abstract

WE have been studying the mechanism of tyrosine amino-transferase (TAT) induction by adrenal steroids in hepatoma (HTC) cell cultures1,2. The hormones increase the rate of enzyme synthesis resulting in an increased intracellular TAT concentration3. The enzyme is continuously turning over with a half-time of degradation of 3 to 7 h (measured as loss of either enzymic or antigenic activity), depending on the experiment and the conditions.

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Author notes

  1. FERDINANDO AURICCHIO

    Present address: Istituto de Patologia Generale, Dell'Universita de Napoli, Napoli, Italy

  2. DAVID MARTIN JUN.

    Present address: Department of Medicine, University of California Medical Center, San Francisco, California, 94122

  3. GORDON TOMKINS

    Present address: Department of Biochemistry, University of California Medical Center, San Francisco, California, 94122

Authors and Affiliations

  1. Laboratory of Molecular Biology, National Institute of Arthritis and Metabolic Diseases, Bethesda, Maryland

    FERDINANDO AURICCHIO, DAVID MARTIN JUN. & GORDON TOMKINS

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  1. FERDINANDO AURICCHIO
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  2. DAVID MARTIN JUN.
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  3. GORDON TOMKINS
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AURICCHIO, F., MARTIN, D. & TOMKINS, G. Control of Degradation and Synthesis of Induced Tyrosine Aminotransferase studied in Hepatoma Cells in Culture. Nature 224, 806–808 (1969). https://doi.org/10.1038/224806b0

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