Abstract
IT is important for our understanding of DNA replication to determine the part played by the cytoplasm in the initiation and maintenance of DNA synthesis in the cell. That cytoplasm can “switch on” DNA synthesis in cells which are in G0 or G1 has been clearly shown in studies of hybrid cells1,2, in transfer experiments using Xenopus egg cytoplasm3,4, and in nuclear transfer experiments using G1 and S phases of the protozoan Stentor5. Whether the G2 nucleus can be “switched on” without first undergoing mitosis is not certain—available evidence is conflicting6–8. The following investigation was carried out on Amoeba proteus to determine (i) if S cytoplasm can initiate synthesis of DNA in the G2 nucleus; (ii) if S cytoplasm can maintain synthesis of DNA indefinitely in “switched on” nuclei; and (iii) if G2 cytoplasm can inhibit DNA synthesis in S nuclei.
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ORD, M. Control of DNA Synthesis in Amoeba proteus. Nature 221, 964–966 (1969). https://doi.org/10.1038/221964a0
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DOI: https://doi.org/10.1038/221964a0
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