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Purification of Influenza Virus in the K-II Zonal Centrifuge

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Abstract

VIRAL material used to prepare inactivated virus vaccines must be concentrated to provide sufficient antigenic mass to stimulate the vaccine's protective mechanisms, and purified to remove nonviral host material which may cause side reactions and sensitization to host (for example, egg) components. A continuous-flow zonal centrifuge, the K-II-B1,2, has been designed for use in large scale production of influenza vaccines. We have grown influenza virus in tissue culture and have concentrated and purified it in a single step process using this centrifuge.

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Author notes

  1. JOHN L. GERIN

    Present address: Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland, 20014

Authors and Affiliations

  1. Department of Infectious Disease Research, Abbott Laboratories, North Chicago, Illinois

    JOHN L. GERIN & NORMAN G. ANDERSON

  2. the Molecular Anatomy Program, Oak Ridge National Laboratory, Oak Ridge, Tennessee

    JOHN L. GERIN & NORMAN G. ANDERSON

Authors
  1. JOHN L. GERIN
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  2. NORMAN G. ANDERSON
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GERIN, J., ANDERSON, N. Purification of Influenza Virus in the K-II Zonal Centrifuge. Nature 221, 1255–1256 (1969). https://doi.org/10.1038/2211255a0

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  • DOI: https://doi.org/10.1038/2211255a0

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