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Quantitative Approach to the Sequential Degradation of Proteins and Peptides

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Abstract

IT has been known for many years that quantitative as well as qualitative information can be derived from the Edman sequential degradation method1,2, but the variable recovery of liberated thiohydantoin derivatives of the constituent amino-acids has made an accurate quantitative analysis of proteins and pep tides difficult. We report here an approach to a quantitative protein degradation method using a volatile Edman reagent (methyl isothiocyanate3), an isotope dilution step for quantitation of the data, and an isotope ratio assay using conventional mass spectrometry4.

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References

  1. Fraenkel-Conrat, H., Harris, J. I., and Levy, A. L., Methods in Biochemical Res., 2, 360 (1952).

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  2. Callewaert, G. L., and Vernon, C. A., Biochem. J., 107, 728 (1968).

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  3. Stepanov, V. M., and Krivtsov, V. F., J. Gen. Chem. (USSR), 35, 53, 556 and 988 (1965).

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  4. Waterfield, M. D., Lovins, R. E., Richards, Frank F., Salomone, R., Smith, G. P., and Haber, E., Fed. Proc., 27, 1341 (1968).

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Author notes

  1. ROBERT E. LOVINS

    Present address: Department of Biochemistry, University of Georgia, Athens, Georgia, 30601

Authors and Affiliations

  1. Department of Internal Medicine, Yale University School of Medicine, New Haven, Connecticut, 06510

    FRANK F. RICHARDS & WILLIAM T. BARNES

  2. Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts, 02139

    ROBERT E. LOVINS & RAMON SALOMONE

  3. Cardiac Unit, Medical Services, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts, 02114

    MICHAEL D. WATERFIELD

Authors
  1. FRANK F. RICHARDS
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  2. WILLIAM T. BARNES
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  3. ROBERT E. LOVINS
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  4. RAMON SALOMONE
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  5. MICHAEL D. WATERFIELD
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RICHARDS, F., BARNES, W., LOVINS, R. et al. Quantitative Approach to the Sequential Degradation of Proteins and Peptides. Nature 221, 1241–1244 (1969). https://doi.org/10.1038/2211241a0

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  • DOI: https://doi.org/10.1038/2211241a0

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