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Polyoma Virus-induced Release of inhibition of Cellular DNA Synthesis caused by Iododeoxyuridine

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Abstract

THE halogenated pyrimidine nucleosides, 5-iodo-2′-deoxyuridine (IUdR) and 5-bromo-2′-deoxyuridine (BUdR), are incorporated instead of thymidine into the DNA of mammalian cells for one division cycle. The synthesis of DNA is then delayed and takes place only in a small fraction of the cells1. At high concentrations of these compounds (or at low concentrations when 5-fluoro-2′-deoxyuridine (FUdR) or amethopterin is present and inhibits endogenous synthesis of thymidine) the DNA doubles once; no further increase in the DNA content of the cultures is observed, even after the analogues have been removed2,3.

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Authors and Affiliations

  1. Department of Microbiology, Research Laboratories, Albert Einstein Medical Center, Philadelphia

    AKIKO KASAMAKI, TAMAR BEN-PORAT & ALBERT S. KAPLAN

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  1. AKIKO KASAMAKI
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  2. TAMAR BEN-PORAT
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  3. ALBERT S. KAPLAN
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KASAMAKI, A., BEN-PORAT, T. & KAPLAN, A. Polyoma Virus-induced Release of inhibition of Cellular DNA Synthesis caused by Iododeoxyuridine. Nature 217, 756–758 (1968). https://doi.org/10.1038/217756a0

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