Abstract
IN a previous communication1 we described a procedure for complete N-methylation of peptide –CONH– groups in oligopeptide derivatives. A mixture of methyl iodide and silver oxide in dimethylformamide was used as the reagent. This reagent is also capable of methylating –OH or –SH groups. It was shown that the N-acyloligopeptide methyl esters exhibited enhanced volatility after methylation, and that the permethylated products were more suitable for mass spectrometric sequential analysis of their amino-acid residues.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Das, B. C., Géro, S. D., and Lederer, E., Biochem. Biophys. Res. Commun., 29, 211 (1967).
Biemann, K., in Mass Spectrometry, chap. 8 (McGraw-Hill, New York, 1962).
Vilkas, E., Miquel, A. M., and Lederer, E., Biochim. Biophys. Acta, 70, 217 (1963).
Barber, M., Jollès, P., Vilkas, E., and Lederer, E., Biochem. Biophys. Res. Commun., 18, 469 (1965).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
DAS, B., GÉRO, S. & LEDERER, E. Detection and Location of N-Methyl-amino-acid Residues in N-Acyloligopeptide Methyl Esters by Mass Spectrometry. Nature 217, 547–548 (1968). https://doi.org/10.1038/217547a0
Received:
Issue Date:
DOI: https://doi.org/10.1038/217547a0
- Springer Nature Limited