Abstract
FOLLICLE stimulating hormone (FSH) was obtained from powdered human pituitary dried with acetone and purified in two ways: (a) by the method of Amir, Barker, Butt and Crooke1, and (b) by the cellosolve extraction procedure of Butt, Crooke and Wolf2 followed by fractionation on DEAE-‘Sephadex A-50’. Haemagglutination inhibition tests showed that luteinizing hormone (LH) and albumin were present to an extent of less than 1 per cent by weight of protein. FSH obtained by both procedures seems to undergo reversible association and dissociation, as the following experiments show.
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References
Amir, S. M., Barker, S. A., Butt, W. R., and Crooke, A. C., Nature, 209, 1092 (1966).
Butt, W. R., Crooke, A. C., and Wolf, A., in Gonadotropins: Physicochemical and Immunological Properties, Ciba Foundation Study Group, No. 22 (edit. by Wolstenholme, G. E. W.), 85 (J. and A. Churchill, Ltd., London, 1965).
Butt, W. R., Crooke, A. C., and Cunningham, F. J., Biochem. J., 81, 596 (1961).
Odell, W. D., Swain, R. W., and Nydick, M., J. Clin. Endocrinol. Metab., 24 (12), 1266 (1964).
Li, C. H., and Starman, B., Nature, 202, 291 (1964).
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GRAY, C. Molecular Weight of Human Follicle Stimulating Hormone. Nature 216, 1112–1113 (1967). https://doi.org/10.1038/2161112a0
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DOI: https://doi.org/10.1038/2161112a0
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