Abstract
THE production of antibodies after exposure to antigens in vitro has been reported1–4 to occur with immune competent cells from nonimmunized animals, but antibody production in response to specific antigens does not seem to have been observed with cultured cells of established human lines. We have now exposed cells of the human lymphoblastoid line P3-J (“Jijoye”), taken from patients with Burkitt's lymphoma, to Coliphage T2 in vitro, and have found that the exposed cells appear to produce a factor capable of neutralizing the phage, probably an antibody. Roswell Park Memorial Institute (RPMI) cell lines5 No. 41 and No. 212, derived from human osteosarcoma and mesothelioma, respectively, produced no such factor.
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KAMEI, H., MOORE, G. Production of Phage-neutralizing Activity in vitro by Cells derived from Burkitt's Lymphoma. Nature 215, 860–861 (1967). https://doi.org/10.1038/215860a0
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DOI: https://doi.org/10.1038/215860a0
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