Abstract
THE inhibitory effect of tris buffer (I)* relative to certain other buffers in a number of biochemical reactions is known1,2. For example, tris gives lower values for the rates of synthesis of proteins in cell-free bacterial preparations than some other buffers. We report here some observations on the inhibition of the reactions of metal complexes of cobalt(III) which may be relevant in assessing the possible inhibitory effects of tris buffer in more complex systems.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Good, N. E., Winger, G. D., Winter, W., Connolly, T. N., Izawa, S., and Singh, R. M. M., Biochemistry, 5, 467 (1967).
O'Sullivan, W. J., and Perrin, D. D., Biochemistry, 3, 18 (1964).
Benesch, R. F., and Benesch, R., Amer. Chem. Soc., 77, 2749 (1955).
Hall, J. L., Swisher, J. A., Brannen, D. G., and Liden, T. M., Inorg. Chem., 1, 409 (1962).
Ahrland, S., Chatt, J., and Davies, N. R., Quart. Rev., 12, 265 (1958).
Mildvan, A. S., and Cohn, M., Biochemistry, 2, 910 (1963).
Nanninga, L. B., J. Phys. Chem., 61, 1144 (1957).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
ALLEN, D., BAKER, D. & GILLARD, R. Metal Complexing by Tris Buffer. Nature 214, 906–907 (1967). https://doi.org/10.1038/214906a0
Received:
Issue Date:
DOI: https://doi.org/10.1038/214906a0
- Springer Nature Limited